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Phenotypic variation of transcriptomic cell types in mouse motor cortex

Nature (London), 2021-10, Vol.598 (7879), p.144-150 [Peer Reviewed Journal]

2020. The Author(s). ;Copyright Nature Publishing Group Oct 7, 2021 ;The Author(s) 2020 ;ISSN: 0028-0836 ;ISSN: 1476-4687 ;EISSN: 1476-4687 ;DOI: 10.1038/s41586-020-2907-3 ;PMID: 33184512

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Title:
Phenotypic variation of transcriptomic cell types in mouse motor cortex
Author: Scala, Federico ; Kobak, Dmitry ; Bernabucci, Matteo ; Bernaerts, Yves ; Cadwell, Cathryn René ; Castro, Jesus Ramon ; Hartmanis, Leonard ; Jiang, Xiaolong ; Laturnus, Sophie ; Miranda, Elanine ; Mulherkar, Shalaka ; Tan, Zheng Huan ; Yao, Zizhen ; Zeng, Hongkui ; Sandberg, Rickard ; Berens, Philipp ; Tolias, Andreas S
Subjects: Animals ; Annotations ; Atlases as Topic ; Biocytin ; Cerebral cortex ; Cortex (motor) ; Cytology ; Datasets ; Electrophysiology ; Female ; GABAergic Neurons - cytology ; GABAergic Neurons - metabolism ; Gene expression ; Gene Expression Profiling ; Gene sequencing ; Glutamates - metabolism ; Lysine - analogs & derivatives ; Lysine - analysis ; Male ; Medicin och hälsovetenskap ; Mice ; Morphology ; Motor Cortex - anatomy & histology ; Motor Cortex - cytology ; Neocortex ; Neurons ; Neurons - classification ; Neurons - cytology ; Neurons - metabolism ; Organ Specificity ; Patch-Clamp Techniques ; Phenotype ; Phenotypes ; Phenotypic variations ; Physiology ; Sequence Analysis, RNA ; Single-Cell Analysis ; Staining and Labeling ; Taxonomy ; Transcriptome ; Transcriptomics
Is Part Of: Nature (London), 2021-10, Vol.598 (7879), p.144-150
Description: Cortical neurons exhibit extreme diversity in gene expression as well as in morphological and electrophysiological properties . Most existing neural taxonomies are based on either transcriptomic or morpho-electric criteria, as it has been technically challenging to study both aspects of neuronal diversity in the same set of cells . Here we used Patch-seq to combine patch-clamp recording, biocytin staining, and single-cell RNA sequencing of more than 1,300 neurons in adult mouse primary motor cortex, providing a morpho-electric annotation of almost all transcriptomically defined neural cell types. We found that, although broad families of transcriptomic types (those expressing Vip, Pvalb, Sst and so on) had distinct and essentially non-overlapping morpho-electric phenotypes, individual transcriptomic types within the same family were not well separated in the morpho-electric space. Instead, there was a continuum of variability in morphology and electrophysiology, with neighbouring transcriptomic cell types showing similar morpho-electric features, often without clear boundaries between them. Our results suggest that neuronal types in the neocortex do not always form discrete entities. Instead, neurons form a hierarchy that consists of distinct non-overlapping branches at the level of families, but can form continuous and correlated transcriptomic and morpho-electrical landscapes within families.
Publisher: England: Nature Publishing Group
Language: English
Identifier: ISSN: 0028-0836
ISSN: 1476-4687
EISSN: 1476-4687
DOI: 10.1038/s41586-020-2907-3
PMID: 33184512
Source: ProQuest One Psychology
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SWEPUB Freely available online
ProQuest Central

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Phenotypic variation of transcriptomic cell types in mouse motor cortex

2020. The Author(s). ;Copyright Nature Publishing Group Oct 7, 2021 ;The Author(s) 2020 ;ISSN: 0028-0836 ;ISSN: 1476-4687 ;EISSN: 1476-4687 ;DOI: 10.1038/s41586-020-2907-3 ;PMID: 33184512

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