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Calcium signaling induced by 15-deoxy-prostamide-J2 promotes cell death by activating PERK, IP3R, and the mitochondrial permeability transition pore

Oncotarget, 2022-12, Vol.13 (1), p.1380-1396

Copyright: © 2022 Ladin et al. ;ISSN: 1949-2553 ;EISSN: 1949-2553 ;DOI: 10.18632/oncotarget.28334 ;PMID: 36580536

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  • Title:
    Calcium signaling induced by 15-deoxy-prostamide-J2 promotes cell death by activating PERK, IP3R, and the mitochondrial permeability transition pore
  • Author: Ladin, Daniel A ; Nelson, Margaret M ; Cota, Estefani ; Colonna, Catherine ; Burns, Colin ; Robidoux, Jacques ; Fisher-Wellman, Kelsey H ; Van Dross-Anderson, Rukiyah
  • Subjects: Apoptosis ; Calcium - metabolism ; Calcium Signaling ; Cell Death ; Humans ; Melanoma ; Mitochondrial Permeability Transition Pore - metabolism ; Prostaglandin D2 - pharmacology ; Research Paper
  • Is Part Of: Oncotarget, 2022-12, Vol.13 (1), p.1380-1396
  • Description: Melanoma is the deadliest form of skin cancer in the US. Although immunotherapeutic checkpoint inhibitors and small-molecule kinase inhibitors have dramatically increased the survival of patients with melanoma, new or optimized therapeutic approaches are still needed to improve outcomes. 15-deoxy-Δ -prostamide J (15d-PMJ ) is an investigational small-molecule that induces ER stress-mediated apoptosis selectively in tumor cells. Additionally, 15d-PMJ2 reduces melanoma growth . To assess the chemotherapeutic potential of 15d-PMJ , the current study sought to uncover molecular pathways by which 15d-PMJ exerts its antitumor activity. B16F10 melanoma and JWF2 squamous cell carcinoma cell lines were cultured in the presence of pharmacological agents that prevent ER or oxidative stress as well as Ca channel blockers to identify mechanisms of 15d-PMJ cell death. Our data demonstrated the ER stress protein, PERK, was required for 15d-PMJ -induced death. PERK activation triggered the release of ER-resident Ca through an IP R sensitive pathway. Increased calcium mobilization led to mitochondrial Ca overload followed by mitochondrial permeability transition pore (mPTP) opening and the deterioration of mitochondrial respiration. Finally, we show the electrophilic double bond located within the cyclopentenone ring of 15d-PMJ was required for its activity. The present study identifies PERK/IP3R/mPTP signaling as a mechanism of 15d-PMJ antitumor activity.
  • Publisher: United States: Impact Journals LLC
  • Language: English
  • Identifier: ISSN: 1949-2553
    EISSN: 1949-2553
    DOI: 10.18632/oncotarget.28334
    PMID: 36580536
  • Source: GFMER Free Medical Journals
    MEDLINE
    PubMed Central
    Alma/SFX Local Collection
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