Language:

An integrated perfusion machine preserves injured human livers for 1 week

Nature biotechnology, 2020-02, Vol.38 (2), p.189-198 [Peer Reviewed Journal]

COPYRIGHT 2020 Nature Publishing Group ;COPYRIGHT 2020 Nature Publishing Group ;2020© The Author(s), under exclusive licence to Springer Nature America, Inc. 2020 ;The Author(s), under exclusive licence to Springer Nature America, Inc. 2020. ;The Author(s), under exclusive licence to Springer Nature America, Inc. 2020 ;ISSN: 1087-0156 ;EISSN: 1546-1696 ;DOI: 10.1038/s41587-019-0374-x ;PMID: 31932726

Full text available

Citations Cited by

Actions
1. Add to My Research
2. Remove from My Research
3. E-mail
4. Print
5. Permalink
6. Citation
7. EasyBib
8. EndNote
9. RefWorks
10. Delicious
11. Export RIS
12. Export BibTeX

Title:
An integrated perfusion machine preserves injured human livers for 1 week
Author: Eshmuminov, Dilmurodjon ; Becker, Dustin ; Bautista Borrego, Lucia ; Hefti, Max ; Schuler, Martin J ; Hagedorn, Catherine ; Muller, Xavier ; Mueller, Matteo ; Onder, Christopher ; Graf, Rolf ; Weber, Achim ; Dutkowski, Philipp ; Rudolf von Rohr, Philipp ; Clavien, Pierre-Alain
Subjects: Adenosine Triphosphate - metabolism ; Alarmins - metabolism ; Animals ; Automation ; Bile ; Biological products ; Biomarkers - metabolism ; Cellular manufacture ; Cellular structure ; Coagulation ; Dextrose ; Electrolytes - metabolism ; Epoprostenol ; Glucagon ; Glucose ; Glucose - metabolism ; Hematocrit ; Hemodynamics ; Hemolysis ; Humans ; In vivo methods and tests ; Insulin ; Liver ; Liver - injuries ; Liver - physiopathology ; Metabolism ; Oxygen - metabolism ; Oxygen Consumption ; Oxygenation ; Perfusion ; Perfusion - instrumentation ; Physiology ; Portal Vein - metabolism ; Preservation, Biological ; Process engineering ; Reperfusion ; Surgical apparatus & instruments ; Swine ; Transplantation ; Veins & arteries ; Viability
Is Part Of: Nature biotechnology, 2020-02, Vol.38 (2), p.189-198
Description: The ability to preserve metabolically active livers ex vivo for 1 week or more could allow repair of poor-quality livers that would otherwise be declined for transplantation. Current approaches for normothermic perfusion can preserve human livers for only 24 h. Here we report a liver perfusion machine that integrates multiple core physiological functions, including automated management of glucose levels and oxygenation, waste-product removal and hematocrit control. We developed the machine in a stepwise fashion using pig livers. Study of multiple ex vivo parameters and early phase reperfusion in vivo demonstrated the viability of pig livers perfused for 1 week without the need for additional blood products or perfusate exchange. We tested the approach on ten injured human livers that had been declined for transplantation by all European centers. After a 7-d perfusion, six of the human livers showed preserved function as indicated by bile production, synthesis of coagulation factors, maintained cellular energy (ATP) and intact liver structure.
Publisher: United States: Nature Publishing Group
Language: English
Identifier: ISSN: 1087-0156
EISSN: 1546-1696
DOI: 10.1038/s41587-019-0374-x
PMID: 31932726
Source: Geneva Foundation Free Medical Journals at publisher websites
MEDLINE
ProQuest Central

Back to results list


INSPIRE LIBRARY - TON DUC THANG UNIVERSITY	(84-028) 37 755 057	Feedback
19 Nguyen Huu Tho St. Dist.7, HCM	thuvien@tdtu.edu.vn	Feedback

An integrated perfusion machine preserves injured human livers for 1 week

Searching Remote Databases, Please Wait