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AS1411 aptamer modified carbon dots via polyethylenimine‐assisted strategy for efficient targeted cancer cell imaging

Cell proliferation, 2020-01, Vol.53 (1), p.e12713-n/a [Peer Reviewed Journal]

2019 The Authors. Published by John Wiley & Sons Ltd. ;2019 The Authors. Cell Proliferation Published by John Wiley & Sons Ltd. ;2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. ;ISSN: 0960-7722 ;EISSN: 1365-2184 ;DOI: 10.1111/cpr.12713 ;PMID: 31691382

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Title:
AS1411 aptamer modified carbon dots via polyethylenimine‐assisted strategy for efficient targeted cancer cell imaging
Author: Kong, Tingting ; Zhou, Ronghui ; Zhang, Yujun ; Hao, Liying ; Cai, Xiaoxiao ; Zhu, Bofeng
Subjects: Aptamers ; Aptamers, Nucleotide - chemistry ; Aptamers, Nucleotide - pharmacokinetics ; Aptamers, Nucleotide - pharmacology ; AS1411 aptamer ; Biocompatibility ; Biosensors ; Cancer ; Capillary electrophoresis ; Carbon ; Carbon - chemistry ; Carbon - pharmacokinetics ; Carbon - pharmacology ; carbon dots ; Cell membranes ; Cholecystokinin ; Confocal microscopy ; Conjugation ; Cytotoxicity ; Deoxyribonucleic acid ; DNA ; Drug Delivery Systems ; Efficiency ; Electrophoresis ; Electrostatic properties ; Flow Cytometry ; Fluorescence ; Fourier transforms ; Humans ; Infrared spectra ; Internalization ; Lasers ; MCF-7 Cells ; Medical imaging ; Microscopy ; Microscopy, Confocal ; Morphology ; Nanomaterials ; Nanoparticles ; Nanotechnology ; Neoplasms - diagnostic imaging ; Neoplasms - metabolism ; nucleolin ; Oligodeoxyribonucleotides - chemistry ; Oligodeoxyribonucleotides - pharmacokinetics ; Oligodeoxyribonucleotides - pharmacology ; Original ; Particle size ; Polyethyleneimine ; Polyethyleneimine - chemistry ; Polyethyleneimine - pharmacokinetics ; Polyethyleneimine - pharmacology ; polyethylenimine ; Quantum dots ; Quantum Dots - chemistry ; Selectivity ; targeted imaging ; Toxicity ; Transmission electron microscopy ; Zeta potential
Is Part Of: Cell proliferation, 2020-01, Vol.53 (1), p.e12713-n/a
Description: Objectives Carbon dots (CDs), as a fascinating class of fluorescent carbon nanomaterials, have been proven to be powerful tools in the field of bioimaging and biosensing due to their small size, suitable photostability and favourable biocompatibility. However, the cellular uptake of free CDs lacks selectivity and the same negative charges as cell membranes may cause inefficient cell internalization. In this study, an efficient detecting and targeting nanosystem was developed based on the DNA aptamer AS1411 modified CDs with polyethyleneimine (PEI) as connecting bridge. Materials and methods Hydrothermally prepared CDs were assembled with positive‐charged PEI, followed by conjugation with AS1411 through electrostatic interaction to form CDs‐PEI‐AS1411 nanocomplexes. The CDs, CDs‐PEI and CDs‐PEI‐AS1411 were characterized by transmission electron microscopy (TEM), fourier transform infrared (FTIR) spectra, UV–vis spectra, zeta potential measurements and capillary electrophoresis characterizations. The cytotoxicity investigation of the CDs‐PEI‐AS1411 and CDs‐PEI in both MCF‐7 and L929 cells was carried out by the CCK‐8 assay. The cellular uptake of the CDs‐PEI‐AS1411 was studied with confocal microscopy and flow cytometry. Results The as‐prepared nanosystem possessed good photostability and no obvious cytotoxicity. On the basis of the confocal laser scanning microscope observation and the flow cytometry studies, the cellular uptake of CDs‐PEI‐AS1411 nanosystem in MCF‐7 cells was significantly higher than that of L929 cells, which revealed the highly selective detection ability of nucleolin‐positive cells. Conclusions The results of this study indicated that the CDs‐PEI‐AS1411 nanosystem had a potential value in cancer cell targeted imaging.
Publisher: England: John Wiley & Sons, Inc
Language: English
Identifier: ISSN: 0960-7722
EISSN: 1365-2184
DOI: 10.1111/cpr.12713
PMID: 31691382
Source: Journals@Ovid Open Access Journal Collection Rolling
MEDLINE
PubMed Central
ProQuest Central
DOAJ Directory of Open Access Journals

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AS1411 aptamer modified carbon dots via polyethylenimine‐assisted strategy for efficient targeted cancer cell imaging

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