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Characterization of feedback-resistant mevalonate kinases from the methanogenic archaeons Methanosaeta concilii and Methanocella paludicola

Microbiology (Society for General Microbiology), 2017-09, Vol.163 (9), p.1283-1291 [Peer Reviewed Journal]

2017 The Authors 2017 ;ISSN: 1350-0872 ;EISSN: 1465-2080 ;DOI: 10.1099/mic.0.000510 ;PMID: 28869407

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Title:
Characterization of feedback-resistant mevalonate kinases from the methanogenic archaeons Methanosaeta concilii and Methanocella paludicola
Author: Kazieva, Ekaterina ; Yamamoto, Yoko ; Tajima, Yoshinori ; Yokoyama, Keiichi ; Katashkina, Joanna ; Nishio, Yousuke
Subjects: Archaea - genetics ; Archaea - metabolism ; Cloning, Molecular ; Enzyme Activation ; Enzyme Inhibitors - pharmacology ; Gene Expression ; Kinetics ; Mevalonic Acid - metabolism ; Phosphorylation ; Phosphotransferases (Alcohol Group Acceptor) - antagonists & inhibitors ; Phosphotransferases (Alcohol Group Acceptor) - genetics ; Phosphotransferases (Alcohol Group Acceptor) - isolation & purification ; Phosphotransferases (Alcohol Group Acceptor) - metabolism ; Plasmids - genetics ; Recombinant Proteins
Is Part Of: Microbiology (Society for General Microbiology), 2017-09, Vol.163 (9), p.1283-1291
Description: The inhibition of mevalonate kinase (MVK) by downstream metabolites is an important mechanism in the regulation of isoprenoid production in a broad range of organisms. The first feedback-resistant MVK was previously discovered in the methanogenic archaeon Methanosarcinamazei. Here, we report the cloning, expression, purification, kinetic characterization and inhibition analysis of MVKs from two other methanogens, Methanosaetaconcilii and Methanocellapaludicola. Similar to the M. mazei MVK, these enzymes were not inhibited by diphosphomevalonate (DPM), dimethylallyl diphosphate (DMAPP), isopentenyldiphosphate (IPP), geranylpyrophosphate (GPP) or farnesylpyrophosphate (FPP). However, they exhibited significantly higher affinity to mevalonate and higher catalytic efficiency than the previously characterized enzyme.
Publisher: England: Microbiology Society
Language: English
Identifier: ISSN: 1350-0872
EISSN: 1465-2080
DOI: 10.1099/mic.0.000510
PMID: 28869407
Source: GFMER Free Medical Journals
MEDLINE
PubMed Central

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Characterization of feedback-resistant mevalonate kinases from the methanogenic archaeons Methanosaeta concilii and Methanocella paludicola

2017 The Authors 2017 ;ISSN: 1350-0872 ;EISSN: 1465-2080 ;DOI: 10.1099/mic.0.000510 ;PMID: 28869407

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