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Dynamics of Translation of Single mRNA Molecules In Vivo

Cell, 2016-05, Vol.165 (4), p.976-989 [Tạp chí có phản biện]

2016 Elsevier Inc. ;Copyright © 2016 Elsevier Inc. All rights reserved. ;2016 The Authors. Published by Elsevier Inc. 2016 Elsevier Inc. ;ISSN: 0092-8674 ;EISSN: 1097-4172 ;DOI: 10.1016/j.cell.2016.04.034 ;PMID: 27153498

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Nhan đề:
Dynamics of Translation of Single mRNA Molecules In Vivo
Tác giả: Yan, Xiaowei ; Hoek, Tim A. ; Vale, Ronald D. ; Tanenbaum, Marvin E.
Chủ đề: 5' Untranslated Regions ; Cell Cycle ; Cell Cycle Proteins - metabolism ; F-Box Proteins - metabolism ; Fluorescence ; Genes, Reporter ; Genetic Techniques ; Green Fluorescent Proteins - analysis ; Humans ; Luminescent Proteins - analysis ; Optical Imaging - methods ; Peptide Chain Elongation, Translational ; Peptide Chain Initiation, Translational ; Protein Biosynthesis ; Red Fluorescent Protein ; Resource ; Ribosomes - metabolism ; RNA, Messenger - chemistry ; RNA, Messenger - metabolism
Là 1 phần của: Cell, 2016-05, Vol.165 (4), p.976-989
Mô tả: Regulation of mRNA translation, the process by which ribosomes decode mRNAs into polypeptides, is used to tune cellular protein levels. Currently, methods for observing the complete process of translation from single mRNAs in vivo are unavailable. Here, we report the long-term (>1 hr) imaging of single mRNAs undergoing hundreds of rounds of translation in live cells, enabling quantitative measurements of ribosome initiation, elongation, and stalling. This approach reveals a surprising heterogeneity in the translation of individual mRNAs within the same cell, including rapid and reversible transitions between a translating and non-translating state. Applying this method to the cell-cycle gene Emi1, we find strong overall repression of translation initiation by specific 5′ UTR sequences, but individual mRNA molecules in the same cell can exhibit dramatically different translational efficiencies. The ability to observe translation of single mRNA molecules in live cells provides a powerful tool to study translation regulation. [Display omitted] •A new imaging method to study dynamics of translation of single mRNAs in vivo•Measurements of ribosome initiation and elongation rates on single mRNA molecules•mRNAs can rapidly switch between a translating and non-translating state•Substantial heterogeneity in ribosome pausing on the Xbp1/Hac1 pause sequence An imaging method is developed for visualizing translation of single mRNA molecules in living cells over long periods of time (>1 hr), revealing a surprising heterogeneity in the translation of mRNA molecules expressed from the same gene in a single cell.
Nơi xuất bản: United States: Elsevier Inc
Ngôn ngữ: English
Số nhận dạng: ISSN: 0092-8674
EISSN: 1097-4172
DOI: 10.1016/j.cell.2016.04.034
PMID: 27153498
Nguồn: Cell Press Free Archives
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Dynamics of Translation of Single mRNA Molecules In Vivo

2016 Elsevier Inc. ;Copyright © 2016 Elsevier Inc. All rights reserved. ;2016 The Authors. Published by Elsevier Inc. 2016 Elsevier Inc. ;ISSN: 0092-8674 ;EISSN: 1097-4172 ;DOI: 10.1016/j.cell.2016.04.034 ;PMID: 27153498

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