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Guidelines for the use of cell lines in biomedical research

British journal of cancer, 2014-09, Vol.111 (6), p.1021-1046 [Peer Reviewed Journal]

Copyright Nature Publishing Group Sep 9, 2014 ;Copyright © 2014 Cancer Research UK 2014 Cancer Research UK ;ISSN: 0007-0920 ;EISSN: 1532-1827 ;DOI: 10.1038/bjc.2014.166 ;PMID: 25117809 ;CODEN: BJCAAI

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Title:
Guidelines for the use of cell lines in biomedical research
Author: Geraghty, R J ; Capes-Davis, A ; Davis, J M ; Downward, J ; Freshney, R I ; Knezevic, I ; Lovell-Badge, R ; Masters, J R W ; Meredith, J ; Stacey, G N ; Thraves, P ; Vias, M
Subjects: Animals ; Biomedical Research - ethics ; Biomedical Research - standards ; Cell Line - classification ; Cell Line - microbiology ; Cryopreservation - standards ; Culture Media - standards ; Equipment and Supplies - standards ; Equipment Contamination - prevention & control ; Genomic Instability ; Guideline ; Humans ; Mycoplasma - isolation & purification ; Phenotype ; Quality Control ; Safety - standards ; Specimen Handling - methods ; Specimen Handling - standards ; United Kingdom
Is Part Of: British journal of cancer, 2014-09, Vol.111 (6), p.1021-1046
Description: Cell-line misidentification and contamination with microorganisms, such as mycoplasma, together with instability, both genetic and phenotypic, are among the problems that continue to affect cell culture. Many of these problems are avoidable with the necessary foresight, and these Guidelines have been prepared to provide those new to the field and others engaged in teaching and instruction with the information necessary to increase their awareness of the problems and to enable them to deal with them effectively. The Guidelines cover areas such as development, acquisition, authentication, cryopreservation, transfer of cell lines between laboratories, microbial contamination, characterisation, instability and misidentification. Advice is also given on complying with current legal and ethical requirements when deriving cell lines from human and animal tissues, the selection and maintenance of equipment and how to deal with problems that may arise.
Publisher: England: Nature Publishing Group
Language: English
Identifier: ISSN: 0007-0920
EISSN: 1532-1827
DOI: 10.1038/bjc.2014.166
PMID: 25117809
CODEN: BJCAAI
Source: GFMER Free Medical Journals
MEDLINE
PubMed Central
ProQuest Central

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Guidelines for the use of cell lines in biomedical research

Copyright Nature Publishing Group Sep 9, 2014 ;Copyright © 2014 Cancer Research UK 2014 Cancer Research UK ;ISSN: 0007-0920 ;EISSN: 1532-1827 ;DOI: 10.1038/bjc.2014.166 ;PMID: 25117809 ;CODEN: BJCAAI

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