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Bromine is an essential trace element for assembly of collagen IV scaffolds in tissue development and architecture

Cell, 2014-06, Vol.157 (6), p.1380-1392 [Peer Reviewed Journal]

Copyright © 2014 Elsevier Inc. All rights reserved. ;2014 Elsevier Inc. All rights reserved. 2014 ;ISSN: 0092-8674 ;EISSN: 1097-4172 ;DOI: 10.1016/j.cell.2014.05.009 ;PMID: 24906154

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Title:
Bromine is an essential trace element for assembly of collagen IV scaffolds in tissue development and architecture
Author: McCall, A Scott ; Cummings, Christopher F ; Bhave, Gautam ; Vanacore, Roberto ; Page-McCaw, Andrea ; Hudson, Billy G
Subjects: Animals ; Basement Membrane - metabolism ; Basement Membrane - ultrastructure ; Bromine - deficiency ; Bromine - metabolism ; Cell Line ; Collagen - metabolism ; Drosophila - growth & development ; Drosophila - metabolism ; Extracellular Matrix Proteins - genetics ; Extracellular Matrix Proteins - metabolism ; Humans ; Imines - metabolism ; Larva - ultrastructure ; Mice ; Peroxidase - genetics ; Peroxidase - metabolism ; Peroxidasin ; Trace Elements - metabolism
Is Part Of: Cell, 2014-06, Vol.157 (6), p.1380-1392
Description: Bromine is ubiquitously present in animals as ionic bromide (Br(-)) yet has no known essential function. Herein, we demonstrate that Br(-) is a required cofactor for peroxidasin-catalyzed formation of sulfilimine crosslinks, a posttranslational modification essential for tissue development and architecture found within the collagen IV scaffold of basement membranes (BMs). Bromide, converted to hypobromous acid, forms a bromosulfonium-ion intermediate that energetically selects for sulfilimine formation. Dietary Br deficiency is lethal in Drosophila, whereas Br replenishment restores viability, demonstrating its physiologic requirement. Importantly, Br-deficient flies phenocopy the developmental and BM defects observed in peroxidasin mutants and indicate a functional connection between Br(-), collagen IV, and peroxidasin. We establish that Br(-) is required for sulfilimine formation within collagen IV, an event critical for BM assembly and tissue development. Thus, bromine is an essential trace element for all animals, and its deficiency may be relevant to BM alterations observed in nutritional and smoking-related disease. PAPERFLICK:
Publisher: United States
Language: English
Identifier: ISSN: 0092-8674
EISSN: 1097-4172
DOI: 10.1016/j.cell.2014.05.009
PMID: 24906154
Source: Cell Press Free Archives
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Bromine is an essential trace element for assembly of collagen IV scaffolds in tissue development and architecture

Copyright © 2014 Elsevier Inc. All rights reserved. ;2014 Elsevier Inc. All rights reserved. 2014 ;ISSN: 0092-8674 ;EISSN: 1097-4172 ;DOI: 10.1016/j.cell.2014.05.009 ;PMID: 24906154

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