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Identification and characterization of a human mitochondrial NAD kinase

Nature communications, 2012-12, Vol.3 (1), p.1248-1248, Article 1248 [Peer Reviewed Journal]

Copyright Nature Publishing Group Dec 2012 ;Copyright © 2012, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. 2012 Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. ;ISSN: 2041-1723 ;EISSN: 2041-1723 ;DOI: 10.1038/ncomms2262 ;PMID: 23212377

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Title:
Identification and characterization of a human mitochondrial NAD kinase
Author: Ohashi, Kazuto ; Kawai, Shigeyuki ; Murata, Kousaku
Subjects: Adenosine Triphosphate - metabolism ; Blotting, Western ; HEK293 Cells - enzymology ; HEK293 Cells - metabolism ; HEK293 Cells - physiology ; Humans ; Microscopy, Fluorescence ; Mitochondria - enzymology ; Mitochondria - metabolism ; Mitochondria - physiology ; Mitochondrial Proteins - metabolism ; Mitochondrial Proteins - physiology ; Phosphotransferases (Alcohol Group Acceptor) - genetics ; Phosphotransferases (Alcohol Group Acceptor) - metabolism ; Phosphotransferases (Alcohol Group Acceptor) - physiology ; Polyphosphates - metabolism ; RNA, Messenger - genetics ; Saccharomyces cerevisiae - metabolism
Is Part Of: Nature communications, 2012-12, Vol.3 (1), p.1248-1248, Article 1248
Description: NAD kinase is the sole NADP(+) biosynthetic enzyme. Despite the great significance of NADP(+), to date no mitochondrial NAD kinase has been identified in human, and the source of human mitochondrial NADP(+) remains elusive. Here we present evidence demonstrating that a human protein of unknown function, C5orf33, is a human mitochondrial NAD kinase; this protein likely represents the missing source of human mitochondrial NADP(+). The C5orf33 protein exhibits NAD kinase activity, utilizing ATP or inorganic polyphosphate, and is localized in the mitochondria of human HEK293A cells. C5orf33 mRNA is more abundant than human cytosolic NAD kinase mRNA in almost all tissues examined. We further show by database searches that some animals and protists carry C5orf33 homologues as their sole NADP(+) biosynthetic enzyme, whereas plants and fungi possess no C5orf33 homologue. These observations provide insights into eukaryotic NADP(+) biosynthesis, which has pivotal roles in cells and organelles.
Publisher: England: Nature Publishing Group
Language: English
Identifier: ISSN: 2041-1723
EISSN: 2041-1723
DOI: 10.1038/ncomms2262
PMID: 23212377
Source: MEDLINE
PubMed Central
ProQuest Central

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Identification and characterization of a human mitochondrial NAD kinase

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