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Analysis of microRNA turnover in mammalian cells following Dicer1 ablation

Nucleic acids research, 2011-07, Vol.39 (13), p.5692-5703 [Peer Reviewed Journal]

The Author(s) 2011. Published by Oxford University Press. 2011 ;ISSN: 0305-1048 ;EISSN: 1362-4962 ;DOI: 10.1093/nar/gkr148 ;PMID: 21447562

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Title:
Analysis of microRNA turnover in mammalian cells following Dicer1 ablation
Author: Gantier, Michael P ; McCoy, Claire E ; Rusinova, Irina ; Saulep, Damien ; Wang, Die ; Xu, Dakang ; Irving, Aaron T ; Behlke, Mark A ; Hertzog, Paul J ; Mackay, Fabienne ; Williams, Bryan R. G
Subjects: Animals ; Cells, Cultured ; DEAD-box RNA Helicases - genetics ; DEAD-box RNA Helicases - metabolism ; Gene Deletion ; HEK293 Cells ; Humans ; Mice ; MicroRNAs - metabolism ; Models, Biological ; Ribonuclease III - genetics ; Ribonuclease III - metabolism ; RNA ; RNA Processing, Post-Transcriptional ; RNA Stability
Is Part Of: Nucleic acids research, 2011-07, Vol.39 (13), p.5692-5703
Description: Although microRNAs (miRNAs) are key regulators of gene expression, little is known of their overall persistence in the cell following processing. Characterization of such persistence is key to the full appreciation of their regulatory roles. Accordingly, we measured miRNA decay rates in mouse embryonic fibroblasts following loss of Dicer1 enzymatic activity. The results confirm the inherent stability of miRNAs, the intracellular levels of which were mostly affected by cell division. Using the decay rates of a panel of six miRNAs representative of the global trend of miRNA decay, we establish a mathematical model of miRNA turnover and determine an average miRNA half-life of 119 h (i.e. ∼5 days). In addition, we demonstrate that select miRNAs turnover more rapidly than others. This study constitutes, to our knowledge, the first in-depth characterization of miRNA decay in mammalian cells. Our findings indicate that miRNAs are up to 10× more stable than messenger RNA and support the existence of novel mechanism(s) controlling selective miRNA cellular concentration and function.
Publisher: England: Oxford University Press
Language: English
Identifier: ISSN: 0305-1048
EISSN: 1362-4962
DOI: 10.1093/nar/gkr148
PMID: 21447562
Source: Geneva Foundation Free Medical Journals at publisher websites
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Open Access: Oxford University Press Open Journals
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Analysis of microRNA turnover in mammalian cells following Dicer1 ablation

The Author(s) 2011. Published by Oxford University Press. 2011 ;ISSN: 0305-1048 ;EISSN: 1362-4962 ;DOI: 10.1093/nar/gkr148 ;PMID: 21447562

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