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GbEXPATR, a species‐specific expansin, enhances cotton fibre elongation through cell wall restructuring

Plant biotechnology journal, 2016-03, Vol.14 (3), p.951-963 [Peer Reviewed Journal]

2015 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd ;2015 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd. ;COPYRIGHT 2016 John Wiley & Sons, Inc. ;2016 Society for Experimental Biology, Association of Applied Biologists and John Wiley & Sons Ltd ;Copyright John Wiley & Sons, Inc. Mar 2016 ;ISSN: 1467-7644 ;EISSN: 1467-7652 ;DOI: 10.1111/pbi.12450 ;PMID: 26269378

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Title:
GbEXPATR, a species‐specific expansin, enhances cotton fibre elongation through cell wall restructuring
Author: Li, Yang ; Tu, Lili ; Pettolino, Filomena A ; Ji, Shengmei ; Hao, Juan ; Yuan, Daojun ; Deng, Fenglin ; Tan, Jiafu ; Hu, Haiyan ; Wang, Qing ; Llewellyn, Danny J ; Zhang, Xianlong
Subjects: Base Sequence ; Breeding ; cell elongation ; Cell Wall - genetics ; Cell Wall - metabolism ; Cell walls ; Cellulose ; Cellulose fibers ; Chitinase ; Cotton ; Cotton Fiber ; Cotton fibers ; cotton fibre ; Crosses, Genetic ; Crystalline cellulose ; Cultivars ; Domestication ; Down-Regulation - genetics ; Elongation ; fiber quality ; Fibers ; Fibre ; Fibres ; Food processing industry ; Gene expression ; Genes ; Genes, Plant ; Genetic Complementation Test ; Genomes ; Genomics ; Gossypium barbadense ; Gossypium barbadense (cotton) ; Gossypium hirsutum ; lint cotton ; Loosening ; Metabolism ; Peptides ; Physiological aspects ; Plant Proteins - chemistry ; Plant Proteins - metabolism ; Plants, Genetically Modified ; Polymorphism, Single Nucleotide - genetics ; Polysaccharides ; Protein Domains ; Proteins ; Quality ; Saccharides ; secondary cell wall ; Sequence Homology, Nucleic Acid ; Solvents ; Species Specificity ; Synthesis ; truncated α‐expansin
Is Part Of: Plant biotechnology journal, 2016-03, Vol.14 (3), p.951-963
Description: Cotton provides us the most important natural fibre. High fibre quality is the major goal of cotton breeding, and introducing genes conferring longer, finer and stronger fibre from Gossypium barbadense to Gossypium hirsutum is an important breeding strategy. We previously analysed the G. barbadense fibre development mechanism by gene expression profiling and found two homoeologous fibre‐specific α‐expansins from G. barbadense, GbEXPA2 and GbEXPATR. GbEXPA2 (from the DT genome) is a classical α‐expansin, while its homoeolog, GbEXPATR (AT genome), encodes a truncated protein lacking the normal C‐terminal polysaccharide‐binding domain of other α‐expansins and is specifically expressed in G. barbadense. Silencing EXPA in G. hirsutum induced shorter fibres with thicker cell walls. GbEXPA2 overexpression in G. hirsutum had no effect on mature fibre length, but produced fibres with a slightly thicker wall and increased crystalline cellulose content. Interestingly, GbEXPATR overexpression resulted in longer, finer and stronger fibres coupled with significantly thinner cell walls. The longer and thinner fibre was associated with lower expression of a number of secondary wall‐associated genes, especially chitinase‐like genes, and walls with lower cellulose levels but higher noncellulosic polysaccharides which advocated that a delay in the transition to secondary wall synthesis might be responsible for better fibre. In conclusion, we propose that α‐expansins play a critical role in fibre development by loosening the cell wall; furthermore, a truncated form, GbEXPATR, has a more dramatic effect through reorganizing secondary wall synthesis and metabolism and should be a candidate gene for developing G. hirsutum cultivars with superior fibre quality.
Publisher: England: Blackwell Pub
Language: English
Identifier: ISSN: 1467-7644
EISSN: 1467-7652
DOI: 10.1111/pbi.12450
PMID: 26269378
Source: Open Access: Wiley Blackwell Open Access Journals

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GbEXPATR, a species‐specific expansin, enhances cotton fibre elongation through cell wall restructuring

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