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P608 Simpleprobe PCR assay for detection of mutations associated with macrolide resistance in mycoplasma genitalium samples

Sexually transmitted infections, 2019-07, Vol.95 (Suppl 1), p.A269 [Peer Reviewed Journal]

Author(s) (or their employer(s)) 2019. No commercial re-use. See rights and permissions. Published by BMJ. ;2019 Author(s) (or their employer(s)) 2019. No commercial re-use. See rights and permissions. Published by BMJ. ;ISSN: 1368-4973 ;EISSN: 1472-3263 ;DOI: 10.1136/sextrans-2019-sti.676

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  • Title:
    P608 Simpleprobe PCR assay for detection of mutations associated with macrolide resistance in mycoplasma genitalium samples
  • Author: Gossé, Marianne ; Lysvand, Hilde ; Pukstad, Brita ; Nordbø, Svein Arne
  • Subjects: Mutation
  • Is Part Of: Sexually transmitted infections, 2019-07, Vol.95 (Suppl 1), p.A269
  • Description: BackgroundMacrolide-resistant strains of Mycoplasma genitalium are an increasing problem throughout the world, and the implementation of a rapid and sensitive assay for mutation detection to guide treatment is needed. Macrolide-resistant strains have been shown to contain base substitutions in positions 2058 and 2059 (Escherichia coli numbering) in region V of the 23S rRNA gene. In this study, we present a SimpleProbe PCR followed by melting curve analysis to differentiate between macrolide-resistant mutants and wild types.MethodsThe assay was performed on 159 Mycoplasma genitalium-positive samples, and the results were compared with DNA sequencing. We also looked at the prevalence of macrolide-resistant strains in a Norwegian population.ResultsOf 139 samples characterized successfully by sequencing, 54 (39%) were wild types and 85 (61%) were mutants, consisting of 59 (42%) A2059G, 24 (17%) A2058G, 1 (1%) A2058T, and 1 (1%) A2059C mutation. The melting curve analysis correctly differentiated between wild-type and mutant strains in all cases, but it could not identify the different mutant types.ConclusionThe SimpleProbe PCR proved to be a simple, rapid, and reliable method for the detection of macrolide-resistant isolates of Mycoplasma genitalium in a clinical setting.DisclosureNo significant relationships.
  • Publisher: London: BMJ Publishing Group LTD
  • Language: English
  • Identifier: ISSN: 1368-4973
    EISSN: 1472-3263
    DOI: 10.1136/sextrans-2019-sti.676
  • Source: ProQuest Central
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