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The steroid hormone 20-hydroxyecdysone binds to dopamine receptor to repress lepidopteran insect feeding and promote pupation

PLoS genetics, 2019-08, Vol.15 (8), p.e1008331-e1008331 [Peer Reviewed Journal]

COPYRIGHT 2019 Public Library of Science ;COPYRIGHT 2019 Public Library of Science ;2019 Kang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. ;2019 Kang et al 2019 Kang et al ;ISSN: 1553-7404 ;ISSN: 1553-7390 ;EISSN: 1553-7404 ;DOI: 10.1371/journal.pgen.1008331 ;PMID: 31412019

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Title:
The steroid hormone 20-hydroxyecdysone binds to dopamine receptor to repress lepidopteran insect feeding and promote pupation
Author: Kang, Xin-Le ; Zhang, Jun-Ying ; Wang, Di ; Zhao, Yu-Meng ; Han, Xiao-Lin ; Wang, Jin-Xing ; Zhao, Xiao-Fan
Palli, Subba Reddy
Subjects: Agricultural pests ; AKT protein ; Amino acids ; Animals ; Apoptosis ; Binding sites ; Biology and Life Sciences ; Body weight ; Calcium (intracellular) ; Cell membranes ; Cell proliferation ; Competition ; Cyclic adenosine monophosphate ; Developmental biology ; Dopamine ; Dopamine - metabolism ; Dopamine Antagonists - pharmacology ; Dopamine receptors ; Ecdysone ; Ecdysterone - metabolism ; Feeding ; Feeding Behavior - drug effects ; Feeding Behavior - physiology ; Flupenthixol ; Flupenthixol - pharmacology ; Food and nutrition ; Food consumption ; G proteins ; Gene expression ; Gene Knockdown Techniques ; Genes ; Hormones ; Insect Proteins - genetics ; Insect Proteins - metabolism ; Insects ; Kinases ; Laboratories ; Larva - drug effects ; Larva - physiology ; Lepidoptera ; Life sciences ; Ligands ; Mammals ; Membrane proteins ; Menopause ; Metamorphosis ; Methods ; Molting ; Molting - drug effects ; Molting - physiology ; Moths - drug effects ; Moths - physiology ; Nervous system ; Phenols (Class of compounds) ; Phosphorylation ; Physical Sciences ; Physiological aspects ; Protein binding ; Proteins ; Pupation ; Receptors, Dopamine - genetics ; Receptors, Dopamine - metabolism ; RNA Interference ; Sf9 Cells ; Software ; Steroid hormones ; Transcription
Is Part Of: PLoS genetics, 2019-08, Vol.15 (8), p.e1008331-e1008331
Description: Holometabolous insects stop feeding at the final larval instar stage and then undergo metamorphosis; however, the mechanism is unclear. In the present study, using the serious lepidopteran agricultural pest Helicoverpa armigera as a model, we revealed that 20-hydroxyecdysone (20E) binds to the dopamine receptor (DopEcR), a G protein-coupled receptor, to stop larval feeding and promote pupation. DopEcR was expressed in various tissues and its level increased during metamorphic molting under 20E regulation. The 20E titer was low during larval feeding stages and high during wandering stages. By contrast, the dopamine (DA) titer was high during larval feeding stages and low during the wandering stages. Injection of 20E or blocking dopamine receptors using the inhibitor flupentixol decreased larval food consumption and body weight. Knockdown of DopEcR repressed larval feeding, growth, and pupation. 20E, via DopEcR, promoted apoptosis; and DA, via DopEcR, induced cell proliferation. 20E opposed DA function by repressing DA-induced cell proliferation and AKT phosphorylation. 20E, via DopEcR, induced gene expression and a rapid increase in intracellular calcium ions and cAMP. 20E induced the interaction of DopEcR with G proteins αs and αq. 20E, via DopEcR, induced protein phosphorylation and binding of the EcRB1-USP1 transcription complex to the ecdysone response element. DopEcR could bind 20E inside the cell membrane or after being isolated from the cell membrane. Mutation of DopEcR decreased 20E binding levels and related cellular responses. 20E competed with DA to bind to DopEcR. The results of the present study suggested that 20E, via binding to DopEcR, arrests larval feeding and promotes pupation.
Publisher: United States: Public Library of Science
Language: English
Identifier: ISSN: 1553-7404
ISSN: 1553-7390
EISSN: 1553-7404
DOI: 10.1371/journal.pgen.1008331
PMID: 31412019
Source: GFMER Free Medical Journals
MEDLINE
PubMed Central
Public Library of Science (PLoS)
ProQuest Central
DOAJ Directory of Open Access Journals

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The steroid hormone 20-hydroxyecdysone binds to dopamine receptor to repress lepidopteran insect feeding and promote pupation

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