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Improved green and red GRAB sensors for monitoring dopaminergic activity in vivo

ISSN: 1548-7091 ;ISSN: 1548-7105 ;EISSN: 1548-7105 ;DOI: 10.1038/s41592-023-02100-w

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Title:
Improved green and red GRAB sensors for monitoring dopaminergic activity in vivo
Author: Zhuo, Yizhou ; Luo, Bin ; Yi, Xinyang ; Dong, Hui ; Miao, Xiaolei ; Wan, Jinxia ; Williams, John T ; Campbell, Malcolm ; Cai, Ruyi ; Qian, Tongrui ; Wang, Lei ; Li, Bozhi ; Li, Fengling ; Weber, Sophia ; Wei, Yu ; Li, Guochuan ; Wang, Huan ; Zheng, Yu ; Zhao, Yulin ; Wolf, Marina ; Zhu, Yingjie ; Watabe-Uchida, Mitsuko ; Yulong, Li
Subjects: Biochemistry ; Biotechnology ; Cell Biology ; Molecular Biology
Description: Dopamine (DA) plays multiple roles in a wide range of physiological and pathological processes via a vast network of dopaminergic projections. To fully dissect the spatiotemporal dynamics of DA release in both dense and sparsely innervated brain regions, we developed a series of green and red fluorescent GPCR activation‒based DA (GRABDA) sensors using a variety of DA receptor subtypes. These sensors have high sensitivity, selectivity, and signal-to-noise properties with subsecond response kinetics and the ability to detect a wide range of DA concentrations. We then used these sensors in freely moving mice to measure both optogenetically evoked and behaviorally relevant DA release while measuring neurochemical signaling in the nucleus accumbens, amygdala, and cortex. Using these sensors, we also detected spatially resolved heterogeneous cortical DA release in mice performing various behaviors. These next-generation GRABDA sensors provide a robust set of tools for imaging dopaminergic activity under a variety of physiological and pathological conditions.
Publisher: Springer Science and Business Media LLC
Creation Date: 2023-11
Language: English
Identifier: ISSN: 1548-7091
ISSN: 1548-7105
EISSN: 1548-7105
DOI: 10.1038/s41592-023-02100-w
Source: Harvard University Library DASH

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Improved green and red GRAB sensors for monitoring dopaminergic activity in vivo

ISSN: 1548-7091 ;ISSN: 1548-7105 ;EISSN: 1548-7105 ;DOI: 10.1038/s41592-023-02100-w

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