skip to main content
Language:
Search Limited to: Search Limited to: Resource type Show Results with: Show Results with: Search type Index

Establishment of Polydopamine-Modified HK-2 Cell Membrane Chromatography and Screening of Active Components from IPlantago asiatica/I L

International journal of molecular sciences, 2024-01, Vol.25 (2) [Peer Reviewed Journal]

COPYRIGHT 2024 MDPI AG ;ISSN: 1422-0067 ;EISSN: 1422-0067 ;DOI: 10.3390/ijms25021153

Full text available

Citations Cited by
  • Title:
    Establishment of Polydopamine-Modified HK-2 Cell Membrane Chromatography and Screening of Active Components from IPlantago asiatica/I L
  • Author: Gao, Hongxue ; Liu, Zhiqiang ; Song, Fengrui ; Xing, Junpeng ; Zheng, Zhong ; Hou, Zong ; Liu, Shu
  • Subjects: Cell membranes ; Chromatography ; Uric acid
  • Is Part Of: International journal of molecular sciences, 2024-01, Vol.25 (2)
  • Description: Cell membrane chromatography (CMC) has been widely recognized as a highly efficient technique for in vitro screening of active compounds. Nevertheless, conventional CMC approaches suffer from a restricted repertoire of cell membrane proteins, making them susceptible to oversaturation. Moreover, the binding mechanism between silica gel and proteins primarily relies on intermolecular hydrogen bonding, which is inherently unstable and somewhat hampers the advancement of CMC. Consequently, this investigation aimed to establish a novel CMC column that could augment protein loading, enhance detection throughput, and bolster binding affinity through the introduction of covalent bonding with proteins. This study utilizes polydopamine (PDA)-coated silica gel, which is formed through the self-polymerization of dopamine (DA), as the carrier for the CMC column filler. The objective is to construct the HK-2/SiO[sub.2]-PDA/CMC model to screen potential therapeutic drugs for gout. To compare the quantity and characteristics of Human Kidney-2 (HK-2) cell membrane proteins immobilized on SiO[sub.2]-PDA and silica gel, the proteins were immobilized on both surfaces. The results indicate that SiO[sub.2]-PDA has a notably greater affinity for membrane proteins compared to silica gel, resulting in a significant improvement in detection efficiency. Furthermore, a screening method utilizing HK-2/SiO[sub.2]-PDA/CMC was utilized to identify seven potential anti-gout compounds derived from Plantago asiatica L. (PAL). The effectiveness of these compounds was further validated using an in vitro cell model of uric acid (UA) reabsorption. In conclusion, this study successfully developed and implemented a novel CMC filler, which has practical implications in the field.
  • Publisher: MDPI AG
  • Language: English
  • Identifier: ISSN: 1422-0067
    EISSN: 1422-0067
    DOI: 10.3390/ijms25021153
  • Source: AUTh Library subscriptions: ProQuest Central
    PubMed Central
    DOAJ Directory of Open Access Journals
Back to results list
Go to Previous pagePrevious Result  11 NextGo to Next page
INSPIRE LIBRARY - TON DUC THANG UNIVERSITY (84-028) 37 755 057 Feedback
19 Nguyen Huu Tho St. Dist.7, HCM thuvien@tdtu.edu.vn

Copyright © 2017 INSPIRE LIBRARY - TON DUC THANG UNIVERSITY

Searching Remote Databases, Please Wait