Language:

Cell Traversal Activity Is Important for Plasmodium falciparum Liver Infection in Humanized Mice

Cell reports (Cambridge), 2017-03, Vol.18 (13), p.3105-3116 [Peer Reviewed Journal]

2017 The Author(s) ;Copyright © 2017 The Author(s). Published by Elsevier Inc. All rights reserved. ;ISSN: 2211-1247 ;EISSN: 2211-1247 ;DOI: 10.1016/j.celrep.2017.03.017 ;PMID: 28355563

Full text available

Citations Cited by

Actions
1. Add to My Research
2. Remove from My Research
3. E-mail
4. Print
5. Permalink
6. Citation
7. EasyBib
8. EndNote
9. RefWorks
10. Delicious
11. Export RIS
12. Export BibTeX

Title:
Cell Traversal Activity Is Important for Plasmodium falciparum Liver Infection in Humanized Mice
Author: Yang, Annie S.P. ; O’Neill, Matthew T. ; Jennison, Charlie ; Lopaticki, Sash ; Allison, Cody C. ; Armistead, Jennifer S. ; Erickson, Sara M. ; Rogers, Kelly L. ; Ellisdon, Andrew M. ; Whisstock, James C. ; Tweedell, Rebecca E. ; Dinglasan, Rhoel R. ; Douglas, Donna N. ; Kneteman, Norman M. ; Boddey, Justin A.
Subjects: Animals ; Cell Movement ; genetics ; hepatocyte ; Hepatocytes - parasitology ; Hepatocytes - pathology ; Humans ; invasion ; Liver - parasitology ; Liver - pathology ; malaria ; Malaria, Falciparum - parasitology ; Malaria, Falciparum - pathology ; Mice, SCID ; motility ; Mutation - genetics ; Parasites - metabolism ; perforin ; Plasmodium falciparum - physiology ; Protozoan Proteins - metabolism ; SPECT ; sporozoite ; Sporozoites - metabolism ; transmission ; virulence
Is Part Of: Cell reports (Cambridge), 2017-03, Vol.18 (13), p.3105-3116
Description: Malaria sporozoites are deposited into the skin by mosquitoes and infect hepatocytes. The molecular basis of how Plasmodium falciparum sporozoites migrate through host cells is poorly understood, and direct evidence of its importance in vivo is lacking. Here, we generated traversal-deficient sporozoites by genetic disruption of sporozoite microneme protein essential for cell traversal (PfSPECT) or perforin-like protein 1 (PfPLP1). Loss of either gene did not affect P. falciparum growth in erythrocytes, in contrast with a previous report that PfPLP1 is essential for merozoite egress. However, although traversal-deficient sporozoites could invade hepatocytes in vitro, they could not establish normal liver infection in humanized mice. This is in contrast with NF54 sporozoites, which infected the humanized mice and developed into exoerythrocytic forms. This study demonstrates that SPECT and perforin-like protein 1 (PLP1) are critical for transcellular migration by P. falciparum sporozoites and demonstrates the importance of cell traversal for liver infection by this human pathogen. [Display omitted] •P. falciparum merozoites do not require PLP1 to egress from erythrocytes•SPECT and PLP1 are critical for P. falciparum sporozoites to traverse human cells•SPECT and PLP1 are dispensable for P. falciparum invasion of human hepatocytes•Host cell traversal is important for P. falciparum to establish liver infection in vivo Malaria sporozoites deposited by a mosquito into the skin must navigate to the liver and infect hepatocytes. Yang et al. reveal the importance of sporozoite transmigration through host cells to establish liver infection in humanized mice by the most virulent human malaria parasite Plasmodium falciparum.
Publisher: United States: Elsevier Inc
Language: English
Identifier: ISSN: 2211-1247
EISSN: 2211-1247
DOI: 10.1016/j.celrep.2017.03.017
PMID: 28355563
Source: Cell Press Free Archives
GFMER Free Medical Journals
MEDLINE
DOAJ Directory of Open Access Journals

Back to results list


INSPIRE LIBRARY - TON DUC THANG UNIVERSITY	(84-028) 37 755 057	Feedback
19 Nguyen Huu Tho St. Dist.7, HCM	thuvien@tdtu.edu.vn	Feedback

Cell Traversal Activity Is Important for Plasmodium falciparum Liver Infection in Humanized Mice

2017 The Author(s) ;Copyright © 2017 The Author(s). Published by Elsevier Inc. All rights reserved. ;ISSN: 2211-1247 ;EISSN: 2211-1247 ;DOI: 10.1016/j.celrep.2017.03.017 ;PMID: 28355563

Searching Remote Databases, Please Wait