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Effect of Honokiol on culture time and survival of Alzheimer’s disease iPSC-derived neurons

Bioimpacts, 2024-01, Vol.14 (1), p.27652-10 [Peer Reviewed Journal]

2024. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. ;2024 The Author(s). 2024 ;ISSN: 2228-5660 ;ISSN: 2228-5652 ;EISSN: 2228-5660 ;DOI: 10.34172/bi.2023.27652

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Title:
Effect of Honokiol on culture time and survival of Alzheimer’s disease iPSC-derived neurons
Author: Le, Duong Thi Thuy ; Vu, Cuong Manh ; Ly, Thuy Thi Bich ; Nguyen, Nam Trung ; Nguyen, Phuong Thi Mai ; Chu, Ha Hoang
Subjects: Alzheimer's disease ; Antioxidants ; Aspartic acid ; Bioactive compounds ; Biological activity ; Biotechnology ; Blood-brain barrier ; Calcium (intracellular) ; Calcium homeostasis ; Caspase-3 ; Cell culture ; Cell death ; Cell survival ; Cerebrospinal fluid ; Disease ; episomal plasmid ; Excitotoxicity ; Fibroblasts ; Fourier transforms ; Gene expression ; honokiol ; induced pluripotent stem cells ; Inflammation ; Inhibitory postsynaptic potentials ; Microglia ; mRNA ; Mutation ; Neurodegeneration ; Neurodegenerative diseases ; neuronal differentiation ; neurotrophic agent ; NMR ; Nuclear magnetic resonance ; Original ; Oxidative stress ; Patients ; pbmcs ; Phenotypes ; Plasmids ; Stem cells ; Toxicity
Is Part Of: Bioimpacts, 2024-01, Vol.14 (1), p.27652-10
Description: Introduction: Patient-derived induced pluripotent stem cells (iPSCs) have been widely used as disease models to test new therapeutic strategies. Moreover, the regenerative potential of stem cells can be improved with the use of biologically active compounds. Our study was designed to explore the effect of honokiol, a small polyphenol molecule extracted from Magnolia officinalis, on the survival and culture time of iPSC-derived neurons from a sporadic Alzheimer’s disease (AD) patient. This study aimed to generate iPSCs from peripheral blood mononuclear cells (PBMCs) of an AD patient using episomal plasmids with a nucleofector system and differentiate them into neurons. These iPSC-derived neurons were used to investigate the effect of honokiol extracted from M. officinalis on their survival and long-term cultures. Methods: IPSCs were generated from PBMCs of an AD patient by introducing Oct-3/4, Sox2, Klf4, L-Myc, and Lin28 using Nucleofector™ Technology. Differentiation of neurons derived from iPSCs was carried out using inducers and recognized by biomarkers. The viability of iPSC-derived neurons with the addition of honokiol extracted from the bark of M. officinalis was determined by the MTT analytical kit. Results: IPSCs were generated by reprogramming AD patient-derived PBMCs and subsequently converted into neurons. The survival and growth of iPSC-derived neurons were significantly enhanced by adding honokiol in the experiment conditions. Conclusion: AD iPSC-derived neurons had a high viability rate when cultured in the presence of honokiol. These results have shown that AD iPSC-derived neurons can be an excellent model for screening neurotrophic agents and improving the conditions for long-term cultures of human iPSC-derived neurons. Honokiol proves to be a potential candidate for cellular therapeutics against neurodegenerative disorders.
Publisher: Tabriz: Tabriz University of Medical Sciences
Language: English
Identifier: ISSN: 2228-5660
ISSN: 2228-5652
EISSN: 2228-5660
DOI: 10.34172/bi.2023.27652
Source: PubMed Central
ROAD: Directory of Open Access Scholarly Resources
ProQuest Central
DOAJ Directory of Open Access Journals

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Effect of Honokiol on culture time and survival of Alzheimer’s disease iPSC-derived neurons

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