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Localization, proteomics, and metabolite profiling reveal a putative vesicular transporter for UDP-glucose

eLife, 2021-07, Vol.10 [Peer Reviewed Journal]

2021, Qian et al. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. ;2021, Qian et al 2021 Qian et al ;ISSN: 2050-084X ;EISSN: 2050-084X ;DOI: 10.7554/eLife.65417 ;PMID: 34269178

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  • Title:
    Localization, proteomics, and metabolite profiling reveal a putative vesicular transporter for UDP-glucose
  • Author: Qian, Cheng ; Wu, Zhaofa ; Sun, Rongbo ; Yu, Huasheng ; Zeng, Jianzhi ; Rao, Yi ; Li, Yulong
  • Subjects: Antibodies ; Endoplasmic reticulum ; Glucose ; Glucose transporter ; Infections ; Localization ; Microscopy ; Neuromodulation ; Neuroscience ; Neurotransmission ; Neurotransmitters ; Physiology ; Proteins ; Proteomics ; solute carrier ; Synaptic vesicles ; transporter ; UDP-glucose
  • Is Part Of: eLife, 2021-07, Vol.10
  • Description: Vesicular neurotransmitter transporters (VNTs) mediate the selective uptake and enrichment of small-molecule neurotransmitters into synaptic vesicles (SVs) and are therefore a major determinant of the synaptic output of specific neurons. To identify novel VNTs expressed on SVs (thus identifying new neurotransmitters and/or neuromodulators), we conducted localization profiling of 361 solute carrier (SLC) transporters tagging with a fluorescent protein in neurons, which revealed 40 possible candidates through comparison with a known SV marker. We parallelly performed proteomics analysis of immunoisolated SVs and identified seven transporters in overlap. Ultrastructural analysis further supported that one of the transporters, SLC35D3, localized to SVs. Finally, by combining metabolite profiling with a radiolabeled substrate transport assay, we identified UDP-glucose as the principal substrate for SLC35D3. These results provide new insights into the functional role of SLC transporters in neurotransmission and improve our understanding of the molecular diversity of chemical transmitters.
  • Publisher: Cambridge: eLife Sciences Publications Ltd
  • Language: English
  • Identifier: ISSN: 2050-084X
    EISSN: 2050-084X
    DOI: 10.7554/eLife.65417
    PMID: 34269178
  • Source: PubMed Central
    ROAD: Directory of Open Access Scholarly Resources
    ProQuest Central
    DOAJ Directory of Open Access Journals
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