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Identification of a C2H2-type zinc finger transcription factor (ZAT10) from Arabidopsis as a substrate of MAP kinase

Plant cell reports, 2012-04, Vol.31 (4), p.737-745 [Tạp chí có phản biện]

Springer-Verlag 2011 ;2015 INIST-CNRS ;ISSN: 0721-7714 ;EISSN: 1432-203X ;DOI: 10.1007/s00299-011-1192-x ;PMID: 22134874 ;CODEN: PCRPD8

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Nhan đề:
Identification of a C2H2-type zinc finger transcription factor (ZAT10) from Arabidopsis as a substrate of MAP kinase
Tác giả: Nguyen, Xuan Canh ; Kim, Sun Ho ; Lee, Kyunghee ; Kim, Kyung Eun ; Liu, Xiao-Min ; Han, Hay Ju ; Hoang, My Hanh Thi ; Lee, Shin-Woo ; Hong, Jong Chan ; Moon, Yong-Hwan ; Chung, Woo Sik
Chủ đề: Amino Acid Sequence ; Arabidopsis - genetics ; Arabidopsis - metabolism ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Biological and medical sciences ; Biomedical and Life Sciences ; Biotechnology ; Cell Biology ; Fundamental and applied biological sciences. Psychology ; Life Sciences ; Mass Spectrometry ; Mitogen-Activated Protein Kinase Kinases - metabolism ; Mitogen-Activated Protein Kinases - metabolism ; Molecular Sequence Data ; Mutagenesis, Site-Directed ; Original Paper ; Phosphopeptides ; Phosphorylation ; Plant Biochemistry ; Plant Sciences ; Protein Interaction Mapping ; Recombinant Proteins ; Sequence Alignment ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Two-Hybrid System Techniques
Là 1 phần của: Plant cell reports, 2012-04, Vol.31 (4), p.737-745
Mô tả: Mitogen-activated protein kinases (MAPKs or MPKs) are one of the most important and conserved signaling molecules in plants. MPKs can directly modulate gene expression by the phosphorylation of transcription factors. However, only a few target substrates of MPKs have been isolated. Here, we identified a C 2 H 2 -type zinc finger transcription factor from Arabidopsis , ZAT10, as a substrate of MPKs. Using in vitro and in vivo protein–protein interaction analyses, we demonstrated that ZAT10 directly interacted with MPK3 and MPK6. ZAT10 was phosphorylated by recombinant Arabidopsis MPK3 and MPK6 in a kinase assay. Furthermore, ZAT10 was also phosphorylated by native MPK3 and MPK6 prepared from Arabidopsis plants in an in-gel kinase assay. Mass spectrometry analysis of phosphopeptides was used to determine two MPK phosphorylation sites in ZAT10. These sites were verified by site-directed mutagenesis and in vitro kinase assays.
Nơi xuất bản: Berlin/Heidelberg: Springer-Verlag
Ngôn ngữ: English
Số nhận dạng: ISSN: 0721-7714
EISSN: 1432-203X
DOI: 10.1007/s00299-011-1192-x
PMID: 22134874
CODEN: PCRPD8
Nguồn: MEDLINE
Alma/SFX Local Collection
ProQuest Central

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Identification of a C2H2-type zinc finger transcription factor (ZAT10) from Arabidopsis as a substrate of MAP kinase

Springer-Verlag 2011 ;2015 INIST-CNRS ;ISSN: 0721-7714 ;EISSN: 1432-203X ;DOI: 10.1007/s00299-011-1192-x ;PMID: 22134874 ;CODEN: PCRPD8

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