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Türkiye'de Yetiştirilen Pekin Ördeklerinde Büyüme Hormonu Geni Polimorfizmi

Tekirdağ Ziraat Fakültesi dergisi, 2022-01, Vol.19 (4), p.798-806

Copyright Namik Kemal University 2022 ;ISSN: 1302-7050 ;EISSN: 1302-7050 ;DOI: 10.33462/jotaf.1089941

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  • Title:
    Türkiye'de Yetiştirilen Pekin Ördeklerinde Büyüme Hormonu Geni Polimorfizmi
  • Author: Eriş, Candan ; Elmaci, Cengiz
  • Subjects: Aquatic birds ; Enzymes ; Gene polymorphism ; Genes ; Genotypes ; Growth hormone ; Growth hormones ; Introns ; Marker-assisted selection ; Polymorphism ; Population (statistical) ; Population studies ; Statistical analysis
  • Is Part Of: Tekirdağ Ziraat Fakültesi dergisi, 2022-01, Vol.19 (4), p.798-806
  • Description: The molecular basis of these traits is revealed by functional genomic methods and provides opportunities to develop genetic improvement programs in farm animals through marker assisted selection (MAS). [...]of the researches, polymorphism was determined only in the fragment amplified from the 2nd intron region. [...]of the digestion of the amplified fragment from the 2nd intron region with BsmF1 restriction enzyme, three genotypes, namely TT (765 bp), CT (765, 593 and 172 bp) and CC (593 and 172 bp) were determined in this region and the frequencies of the T and C alleles were calculated as 0.75 and 0.25, respectively. Since the difference between the values observed in the 2nd intron region of the growth hormone gene and the expected values in the studied population was not statistically significant (P>0.05), it was understood that the population in studied was in Hardy-Weinberg equilibrium. PCR products belonging to the 442 bp and 1378 bp regions of the 3rd and 4th introns of the growth hormone gene, respectively, were also treated with BsmF1 restriction enzyme, but the cleavage region of this enzyme was not found, so there was only one genotype was obtained in the 3rd and 4th introns of the studied population. [...]it was understood that these two regions are monomorphic in terms of BsmF1 locus.
  • Publisher: Tekirdag: Namik Kemal University
  • Language: Turkish
  • Identifier: ISSN: 1302-7050
    EISSN: 1302-7050
    DOI: 10.33462/jotaf.1089941
  • Source: ProQuest Central
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