Language:

Amperometric post spike feet reveal most exocytosis is via extended kiss-and-run fusion

Scientific reports, 2012, Vol.2 (1), p.907-907, Article 907 [Peer Reviewed Journal]

Copyright Nature Publishing Group Nov 2012 ;Copyright © 2012, Macmillan Publishers Limited. All rights reserved 2012 Macmillan Publishers Limited. All rights reserved ;ISSN: 2045-2322 ;EISSN: 2045-2322 ;DOI: 10.1038/srep00907 ;PMID: 23205269

Full text available

Citations Cited by

Actions
1. Add to My Research
2. Remove from My Research
3. E-mail
4. Print
5. Permalink
6. Citation
7. EasyBib
8. EndNote
9. RefWorks
10. Delicious
11. Export RIS
12. Export BibTeX

Title:
Amperometric post spike feet reveal most exocytosis is via extended kiss-and-run fusion
Author: Mellander, Lisa J ; Trouillon, Raphaël ; Svensson, Maria I ; Ewing, Andrew G
Subjects: Action Potentials ; Amperometry ; Animals ; Catecholamines ; Cell Communication - drug effects ; Cell Membrane - drug effects ; Cell Membrane - metabolism ; Chemical Sciences ; chromaffin cells ; events ; Exocytosis ; Exocytosis - drug effects ; Exocytosis - physiology ; Feet ; Kemi ; Kinetics ; Lipids ; Liposomes - chemistry ; Liposomes - pharmacology ; Membrane Fusion - drug effects ; Membrane vesicles ; Microelectrodes ; Neurotransmitter Agents - metabolism ; PC12 Cells ; Pheochromocytoma cells ; Phospholipids - chemistry ; Phospholipids - pharmacology ; pore ; Rats ; release ; Secretory Vesicles - drug effects ; Secretory Vesicles - metabolism ; Synaptic Potentials - drug effects ; Synaptic Transmission - drug effects ; Transmitters ; Vesicle fusion
Is Part Of: Scientific reports, 2012, Vol.2 (1), p.907-907, Article 907
Description: The basis for communication between nerve cells lies in the process of exocytosis, the fusion of neurotransmitter filled vesicles with the cell membrane resulting in release of the signaling molecules. Even though much is known about this process, the extent that the vesicles are emptied upon fusion is a topic that is being debated. We have analyzed amperometric peaks corresponding to release at PC12 cells and find stable plateau currents during the decay of peaks, indicating closing of the vesicle after incomplete release of its content. Using lipid incubations to alter the amount of transmitter released we were able to estimate the initial vesicular content, and from that, the fraction of release. We propose a process for most exocytosis events where the vesicle partially opens to release transmitter and then closes directly again, leaving the possibility for regulation of transmission within events.
Publisher: England: Nature Publishing Group
Language: English
Identifier: ISSN: 2045-2322
EISSN: 2045-2322
DOI: 10.1038/srep00907
PMID: 23205269
Source: PubMed (Medline)
MEDLINE
SWEPUB Freely available online
ProQuest Central
DOAJ Directory of Open Access Journals

Back to results list


INSPIRE LIBRARY - TON DUC THANG UNIVERSITY	(84-028) 37 755 057	Feedback
19 Nguyen Huu Tho St. Dist.7, HCM	thuvien@tdtu.edu.vn	Feedback

Amperometric post spike feet reveal most exocytosis is via extended kiss-and-run fusion

Copyright Nature Publishing Group Nov 2012 ;Copyright © 2012, Macmillan Publishers Limited. All rights reserved 2012 Macmillan Publishers Limited. All rights reserved ;ISSN: 2045-2322 ;EISSN: 2045-2322 ;DOI: 10.1038/srep00907 ;PMID: 23205269

Searching Remote Databases, Please Wait