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HPLC method for the quantitative determination of hexeneuronic acid groups in chemical pulps
Carbohydrate research, 1996-11, Vol.294 (1-4), p.41-51
[Peer Reviewed Journal]
ISSN: 0008-6215 ;ISSN: 1873-426X ;EISSN: 1873-426X ;DOI: 10.1016/s0008-6215(96)00198-x ;PMID: 8962485
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Title:
HPLC method for the quantitative determination of hexeneuronic acid groups in chemical pulps
Author:
Gellerstedt, G
;
Li, J
Subjects:
4-deoxy-L-threo-hex-4-enopyranosyluronic acid
;
chemical composition
;
chemical reaction mechanisms
;
chemical reactions
;
Chromatography, High Pressure Liquid - methods
;
Colorimetry
;
Glucuronates - metabolism
;
Hexuronic Acids - analysis
;
high performance liquid chromatography
;
Hydrolysis
;
Kinetics
;
kraft pulping
;
kraft pulps
;
Mercury - metabolism
;
Molecular Structure
;
Periodic Acid - metabolism
;
PINE KRAFT PULP
;
Pyruvates - analysis
;
Pyruvates - metabolism
;
quantitative analysis
;
RESIDUES
;
TECHNOLOGY
;
TEKNIKVETENSKAP
;
Temperature
;
Thiobarbiturates - metabolism
;
uronic acids
;
Wood
;
wood pulp
;
xylan
;
Xylans - metabolism
Is Part Of:
Carbohydrate research, 1996-11, Vol.294 (1-4), p.41-51
Description:
It has recently been demonstrated that 4-deoxy-L-threo-hex-4-enopyranosyluronic acid ("hexeneuronic acid") is present in kraft pulps and linked to the xylan backbone. An analytical method for the quantitative determination of hexeneuronic acid groups has now been developed. The procedure involves a selective hydrolysis with mercuric acetate of the glucosidic linkage between the hexeneuronic acid group and the xylan chain, followed by oxidation with periodate to form beta-formyl pyruvic acid. The latter is reacted with thiobarbituric acid, and the red-coloured adduct formed is separated by reverse phase HPLC and quantified by measuring the absorbance at 549 nm. Some kraft pulps have been analysed to illustrate the contribution of hexeneuronic acid groups to the total amount of oxidizable structures present in such pulps.
Publisher:
Netherlands
Language:
English
Identifier:
ISSN: 0008-6215
ISSN: 1873-426X
EISSN: 1873-426X
DOI: 10.1016/s0008-6215(96)00198-x
PMID: 8962485
Source:
MEDLINE
Alma/SFX Local Collection
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