Language:

Tanshinone II A enhances pyroptosis and represses cell proliferation of HeLa cells by regulating miR-145/GSDMD signaling pathway

Bioscience reports, 2020-04, Vol.40 (4) [Peer Reviewed Journal]

2020 The Author(s). ;Copyright Springer Nature B.V. Apr 2020 ;2020 The Author(s). 2020 ;ISSN: 0144-8463 ;EISSN: 1573-4935 ;DOI: 10.1042/BSR20200259 ;PMID: 32232409

Full text available

Citations Cited by

Actions
1. Add to My Research
2. Remove from My Research
3. E-mail
4. Print
5. Permalink
6. Citation
7. EasyBib
8. EndNote
9. RefWorks
10. Delicious
11. Export RIS
12. Export BibTeX

Title:
Tanshinone II A enhances pyroptosis and represses cell proliferation of HeLa cells by regulating miR-145/GSDMD signaling pathway
Author: Tong, Wenjuan ; Guo, Jianghong ; Yang, Chunfen
Subjects: 1-Phosphatidylinositol 3-kinase ; Abietanes - pharmacology ; Abietanes - therapeutic use ; AKT protein ; Anticancer properties ; Antitumor activity ; Apoptosis ; Cancer ; Cell Death & Injury ; Cell growth ; Cell proliferation ; Cell Proliferation - drug effects ; Cell Proliferation - genetics ; Cervical cancer ; Cervix ; Chemical Biology ; Drug therapy ; Female ; Gene Expression Regulation, Neoplastic - drug effects ; HeLa Cells ; Humans ; Intracellular Signaling Peptides and Proteins - genetics ; MicroRNAs - genetics ; MicroRNAs - metabolism ; miRNA ; NF-κB protein ; Pathogenesis ; Pharmacology & Toxicology ; Phosphate-Binding Proteins - genetics ; Plasmids ; Pyroptosis - drug effects ; Ribonucleic acid ; RNA ; Signal transduction ; Signal Transduction - drug effects ; Signal Transduction - genetics ; Signaling ; Transfection ; Uterine Cervical Neoplasms - drug therapy ; Uterine Cervical Neoplasms - genetics ; Uterine Cervical Neoplasms - pathology
Is Part Of: Bioscience reports, 2020-04, Vol.40 (4)
Description: Cervical cancer is the fourth most common cancer in women globally. Lack of effective pharmacotherapies for cervical cancer mainly attributed to an elusive understanding of the mechanism underlying its pathogenesis. Pyroptosis plays a key role in inflammation and cancer. Our study identified microRNA (miR) 145 (miR-145)/gasdermin D (GSDMD) signaling pathway as critical mediators in the effect of tanshinone II A on HeLa cells. In the present study, we found that treatment of tanshinone II A led to an obvious repression of cell proliferation and an increase in apoptosis on HeLa cells, especially in high concentration. Compared with the controlled group, tanshinone II A enhanced the activity of caspase3 and caspase9. Notably, the results demonstrated that tanshinone II A regulated cell proliferation of HeLa cells by regulating miR-145/GSDMD signaling pathway. Treatment of tanshinone II A significantly up-regulated the expression of GSDMD and miR-145. After transfection of si-miR-145 plasmids, the effects of tanshinone II A on HeLa cells were converted, including cell proliferation, apoptosis and pyroptosis. In addition, the results showed that tanshinone II A treatment altered the expression level of PI3K, p-Akt, NF-kB p65 and Lc3-I. Collectively, our findings demonstrate that tanshinone II A exerts anticancer activity on HeLa cells by regulating miR-145/GSDMD signaling. The present study is the first time to identify miR-145 as a candidate target in cervical cancer and show an association between miR-145 and pyroptosis, which provides a novel therapy for the treatment of cervical cancer.
Publisher: England: Springer Nature B.V
Language: English;Chinese
Identifier: ISSN: 0144-8463
EISSN: 1573-4935
DOI: 10.1042/BSR20200259
PMID: 32232409
Source: Geneva Foundation Free Medical Journals
Open Access: PubMed Central
MEDLINE

Back to results list


INSPIRE LIBRARY - TON DUC THANG UNIVERSITY	(84-028) 37 755 057	Feedback
19 Nguyen Huu Tho St. Dist.7, HCM	thuvien@tdtu.edu.vn	Feedback

Tanshinone II A enhances pyroptosis and represses cell proliferation of HeLa cells by regulating miR-145/GSDMD signaling pathway

2020 The Author(s). ;Copyright Springer Nature B.V. Apr 2020 ;2020 The Author(s). 2020 ;ISSN: 0144-8463 ;EISSN: 1573-4935 ;DOI: 10.1042/BSR20200259 ;PMID: 32232409

Searching Remote Databases, Please Wait