Language:

Human Intestinal Enteroids as a Model System of Shigella Pathogenesis

Infection and immunity, 2019-04, Vol.87 (4) [Peer Reviewed Journal]

Copyright © 2019 American Society for Microbiology. ;Copyright © 2019 American Society for Microbiology. 2019 American Society for Microbiology ;ISSN: 0019-9567 ;EISSN: 1098-5522 ;DOI: 10.1128/IAI.00733-18 ;PMID: 30642906

Full text available

Citations Cited by

Actions
1. Add to My Research
2. Remove from My Research
3. E-mail
4. Print
5. Permalink
6. Citation
7. EasyBib
8. EndNote
9. RefWorks
10. Delicious
11. Export RIS
12. Export BibTeX

Title:
Human Intestinal Enteroids as a Model System of Shigella Pathogenesis
Author: Koestler, Benjamin J ; Ward, Cara M ; Fisher, C R ; Rajan, Anubama ; Maresso, Anthony W ; Payne, Shelley M
Young, Vincent B.
Subjects: Cell Culture Techniques ; Cellular Microbiology: Pathogen-Host Cell Molecular Interactions ; Dysentery, Bacillary - genetics ; Dysentery, Bacillary - metabolism ; Dysentery, Bacillary - microbiology ; Humans ; Intestinal Mucosa - cytology ; Intestinal Mucosa - metabolism ; Intestinal Mucosa - microbiology ; Large Neutral Amino Acid-Transporter 1 - genetics ; Large Neutral Amino Acid-Transporter 1 - metabolism ; Models, Biological ; Organoids - growth & development ; Organoids - metabolism ; Organoids - microbiology ; Shigella flexneri - pathogenicity ; Shigella flexneri - physiology ; Spotlight ; Stem Cells - cytology ; Stem Cells - microbiology ; Virulence
Is Part Of: Infection and immunity, 2019-04, Vol.87 (4)
Description: The enteric bacterium and intracellular human pathogen causes hundreds of millions of cases of the diarrheal disease shigellosis per year worldwide. is acquired by ingestion of contaminated food or water; upon reaching the colon, the bacteria invade colonic epithelial cells, replicate intracellularly, spread to adjacent cells, and provoke an intense inflammatory response. There is no animal model that faithfully recapitulates human disease; thus, cultured cells have been used to model pathogenesis. However, the use of transformed cells in culture does not provide the same environment to the bacteria as the normal human intestinal epithelium. Recent advances in tissue culture now enable the cultivation of human intestinal enteroids (HIEs), which are derived from human intestinal stem cells, grown , and then differentiated into "mini-intestines." Here, we demonstrate that HIEs can be used to model pathogenesis. We show that invades polarized HIE monolayers preferentially via the basolateral surface. After invades HIE monolayers, replicates within HIE cells and forms actin tails. also increases the expression of HIE proinflammatory signals and the amino acid transporter SLC7A5. Finally, we demonstrate that disruption of HIE tight junctions enables invasion via the apical surface.
Publisher: United States: American Society for Microbiology
Language: English
Identifier: ISSN: 0019-9567
EISSN: 1098-5522
DOI: 10.1128/IAI.00733-18
PMID: 30642906
Source: Geneva Foundation Free Medical Journals
MEDLINE
PubMed Central

Back to results list


INSPIRE LIBRARY - TON DUC THANG UNIVERSITY	(84-028) 37 755 057	Feedback
19 Nguyen Huu Tho St. Dist.7, HCM	thuvien@tdtu.edu.vn	Feedback

Human Intestinal Enteroids as a Model System of Shigella Pathogenesis

Copyright © 2019 American Society for Microbiology. ;Copyright © 2019 American Society for Microbiology. 2019 American Society for Microbiology ;ISSN: 0019-9567 ;EISSN: 1098-5522 ;DOI: 10.1128/IAI.00733-18 ;PMID: 30642906

Searching Remote Databases, Please Wait