Language:

Transgenic Clustered Regularly Interspaced Short Palindromic Repeat/Cas9-Mediated Viral Gene Targeting for Antiviral Therapy of Bombyx mori Nucleopolyhedrovirus

Journal of virology, 2017-04, Vol.91 (8) [Peer Reviewed Journal]

Copyright © 2017 Chen et al. ;Copyright © 2017 Chen et al. 2017 Chen et al. ;ISSN: 0022-538X ;EISSN: 1098-5514 ;DOI: 10.1128/JVI.02465-16 ;PMID: 28122981

Full text available

Citations Cited by

Actions
1. Add to My Research
2. Remove from My Research
3. E-mail
4. Print
5. Permalink
6. Citation
7. EasyBib
8. EndNote
9. RefWorks
10. Delicious
11. Export RIS
12. Export BibTeX

Title:
Transgenic Clustered Regularly Interspaced Short Palindromic Repeat/Cas9-Mediated Viral Gene Targeting for Antiviral Therapy of Bombyx mori Nucleopolyhedrovirus
Author: Chen, Shuqing ; Hou, Chengxiang ; Bi, Honglun ; Wang, Yueqiang ; Xu, Jun ; Li, Muwang ; James, Anthony A ; Huang, Yongping ; Tan, Anjiang
McFadden, Grant
Subjects: Animals ; Animals, Genetically Modified ; Antiviral Agents - metabolism ; Baculoviridae ; Baculoviridae - genetics ; Baculoviridae - growth & development ; Bombyx - immunology ; Bombyx - physiology ; Bombyx - virology ; Bombyx mori ; Bombyx mori NPV ; Cellular Response to Infection ; CRISPR-Cas Systems ; Disease Resistance ; DNA, Viral - metabolism ; Microbial Viability ; Mutagenesis ; Sequence Deletion
Is Part Of: Journal of virology, 2017-04, Vol.91 (8)
Description: We developed a novel antiviral strategy by combining transposon-based transgenesis and the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) system for the direct cleavage of Bombyx mori nucleopolyhedrovirus (BmNPV) genome DNA to promote virus clearance in silkworms. We demonstrate that transgenic silkworms constitutively expressing Cas9 and guide RNAs targeting the BmNPV ( ) and genes effectively induce target-specific cleavage and subsequent mutagenesis, especially large (∼7-kbp) segment deletions in BmNPV genomes, and thus exhibit robust suppression of BmNPV proliferation. Transgenic animals exhibited higher and inheritable resistance to BmNPV infection than wild-type animals. Our approach will not only contribute to modern sericulture but also shed light on future antiviral therapy. Pathogen genome targeting has shown its potential in antiviral research. However, transgenic CRISPR/Cas9 system-mediated viral genome targeting has not been reported as an antiviral strategy in a natural animal host of a virus. Our data provide an effective approach against BmNPV infection in a real-world biological system and demonstrate the potential of transgenic CRISPR/Cas9 systems in antiviral research in other species.
Publisher: United States: American Society for Microbiology
Language: English
Identifier: ISSN: 0022-538X
EISSN: 1098-5514
DOI: 10.1128/JVI.02465-16
PMID: 28122981
Source: MEDLINE
PubMed Central

Back to results list


INSPIRE LIBRARY - TON DUC THANG UNIVERSITY	(84-028) 37 755 057	Feedback
19 Nguyen Huu Tho St. Dist.7, HCM	thuvien@tdtu.edu.vn	Feedback

Transgenic Clustered Regularly Interspaced Short Palindromic Repeat/Cas9-Mediated Viral Gene Targeting for Antiviral Therapy of Bombyx mori Nucleopolyhedrovirus

Copyright © 2017 Chen et al. ;Copyright © 2017 Chen et al. 2017 Chen et al. ;ISSN: 0022-538X ;EISSN: 1098-5514 ;DOI: 10.1128/JVI.02465-16 ;PMID: 28122981

Searching Remote Databases, Please Wait