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Non-mulberry Silk Fibroin Biomaterial for Corneal Regeneration

Scientific reports, 2016-02, Vol.6 (1), p.21840-21840, Article 21840 [Peer Reviewed Journal]

Copyright Nature Publishing Group Feb 2016 ;Copyright © 2016, Macmillan Publishers Limited 2016 Macmillan Publishers Limited ;ISSN: 2045-2322 ;EISSN: 2045-2322 ;DOI: 10.1038/srep21840 ;PMID: 26908015

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Title:
Non-mulberry Silk Fibroin Biomaterial for Corneal Regeneration
Author: Hazra, Sarbani ; Nandi, Sudip ; Naskar, Deboki ; Guha, Rajdeep ; Chowdhury, Sushovan ; Pradhan, Nirparaj ; Kundu, Subhas C ; Konar, Aditya
Subjects: Amnion - transplantation ; Amniotic membrane ; Animals ; Antheraea mylitta ; ATP Binding Cassette Transporter, Sub-Family G, Member 2 - metabolism ; Biocompatible Materials - chemistry ; Biocompatible Materials - pharmacology ; Cell Adhesion - drug effects ; Cell growth ; Cell migration ; Cell Movement - drug effects ; Cell Proliferation - drug effects ; Cells, Cultured ; Cornea ; Cornea - pathology ; Cornea - physiology ; Cornea - ultrastructure ; Corneal Keratocytes - cytology ; Corneal Keratocytes - drug effects ; Corneal Keratocytes - metabolism ; Electroretinography ; Epithelial cells ; Explants ; Fibroins - chemistry ; Fibroins - pharmacology ; Inflammation ; Intraocular Pressure - physiology ; Keratin-3 - metabolism ; Leukocyte migration ; Microscopy, Electron, Scanning ; Microscopy, Fluorescence ; Moths - metabolism ; Rabbits ; Rats ; Rats, Sprague-Dawley ; Refractometry ; Regeneration - drug effects ; Silk ; Stem cells ; Vascularization ; Vimentin ; Vimentin - metabolism
Is Part Of: Scientific reports, 2016-02, Vol.6 (1), p.21840-21840, Article 21840
Description: Successful repair of a damaged corneal surface is a great challenge and may require the use of a scaffold that supports cell growth and differentiation. Amniotic membrane is currently used for this purpose, in spite of its limitations. A thin transparent silk fibroin film from non-mulberry Antheraea mylitta (Am) has been developed which offers to be a promising alternative. The silk scaffolds provide sufficient rigidity for easy handling, the scaffolds support the sprouting, migration, attachment and growth of epithelial cells and keratocytes from rat corneal explants; the cells form a cell sheet, preserve their phenotypes, express cytokeratin3 and vimentin respectively. The films also support growth of limbal stem cell evidenced by expression of ABCG2. The cell growth on the silk film and the amniotic membrane is comparable. The implanted film within the rabbit cornea remains transparent, stable. The clinical examination as well as histology shows absence of any inflammatory response or neovascularization. The corneal surface integrity is maintained; tear formation, intraocular pressure and electroretinography of implanted eyes show no adverse changes. The silk fibroin film from non-mulberry silk worms may be a worthy candidate for use as a corneal scaffold.
Publisher: England: Nature Publishing Group
Language: English
Identifier: ISSN: 2045-2322
EISSN: 2045-2322
DOI: 10.1038/srep21840
PMID: 26908015
Source: MEDLINE
PubMed Central
ProQuest Central
DOAJ Directory of Open Access Journals

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Non-mulberry Silk Fibroin Biomaterial for Corneal Regeneration

Copyright Nature Publishing Group Feb 2016 ;Copyright © 2016, Macmillan Publishers Limited 2016 Macmillan Publishers Limited ;ISSN: 2045-2322 ;EISSN: 2045-2322 ;DOI: 10.1038/srep21840 ;PMID: 26908015

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