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Extensive Degradation of RNA Precursors by the Exosome in Wild-Type Cells

Molecular cell, 2012-11, Vol.48 (3), p.409-421 [Peer Reviewed Journal]

2012 Elsevier Inc. ;Copyright © 2012 Elsevier Inc. All rights reserved. ;Distributed under a Creative Commons Attribution 4.0 International License ;2012 Elsevier Inc. All rights reserved. 2012 ;ISSN: 1097-2765 ;EISSN: 1097-4164 ;DOI: 10.1016/j.molcel.2012.08.018 ;PMID: 23000176

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Title:
Extensive Degradation of RNA Precursors by the Exosome in Wild-Type Cells
Author: Gudipati, Rajani Kanth ; Xu, Zhenyu ; Lebreton, Alice ; Séraphin, Bertrand ; Steinmetz, Lars M. ; Jacquier, Alain ; Libri, Domenico
Subjects: Bacteriology ; Biochemistry, Molecular Biology ; Blotting, Northern ; Cellular Biology ; degradation ; Exosome Multienzyme Ribonuclease Complex ; Exosome Multienzyme Ribonuclease Complex - genetics ; Exosome Multienzyme Ribonuclease Complex - metabolism ; exosomes ; Gene Expression Profiling ; genes ; Genomics ; Introns ; Introns - genetics ; Life Sciences ; Microbiology and Parasitology ; Molecular biology ; mutants ; Mutation ; non-coding RNA ; Oligonucleotide Array Sequence Analysis ; protein subunits ; Quantitative Methods ; ribosomal RNA ; RNA Precursors ; RNA Precursors - genetics ; RNA Precursors - metabolism ; RNA Processing, Post-Transcriptional ; RNA, Fungal ; RNA, Fungal - genetics ; RNA, Fungal - metabolism ; RNA, Ribosomal ; RNA, Ribosomal - genetics ; RNA, Ribosomal - metabolism ; RNA, Small Nucleolar ; RNA, Small Nucleolar - genetics ; RNA, Small Nucleolar - metabolism ; RNA, Transfer ; RNA, Transfer - genetics ; RNA, Transfer - metabolism ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - metabolism ; Saccharomyces cerevisiae Proteins ; Saccharomyces cerevisiae Proteins - genetics ; Saccharomyces cerevisiae Proteins - metabolism ; Subcellular Processes ; Substrate Specificity ; transcriptome ; transfer RNA
Is Part Of: Molecular cell, 2012-11, Vol.48 (3), p.409-421
Description: The exosome is a complex involved in the maturation of rRNA and sn-snoRNA, in the degradation of short-lived noncoding RNAs, and in the quality control of RNAs produced in mutants. It contains two catalytic subunits, Rrp6p and Dis3p, whose specific functions are not fully understood. We analyzed the transcriptome of combinations of Rrp6p and Dis3p catalytic mutants by high-resolution tiling arrays. We show that Dis3p and Rrp6p have both overlapping and specific roles in degrading distinct classes of substrates. We found that transcripts derived from more than half of intron-containing genes are degraded before splicing. Surprisingly, we also show that the exosome degrades large amounts of tRNA precursors despite the absence of processing defects. These results underscore the notion that large amounts of RNAs produced in wild-type cells are discarded before entering functional pathways and suggest that kinetic competition with degradation proofreads the efficiency and accuracy of processing. ► Genome-wide analyses reveal specific function of the exosome catalytic subunits ► Most intron-containing pre-mRNAs are degraded in splicing-proficient cells ► Large amounts of tRNA precursors are degraded by the exosome in wild-type cells ► Kinetic competition with degradation promotes quality control of RNA processing
Publisher: United States: Elsevier Inc
Language: English
Identifier: ISSN: 1097-2765
EISSN: 1097-4164
DOI: 10.1016/j.molcel.2012.08.018
PMID: 23000176
Source: MEDLINE
Open Access: Cell Press Free Archives

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Extensive Degradation of RNA Precursors by the Exosome in Wild-Type Cells

2012 Elsevier Inc. ;Copyright © 2012 Elsevier Inc. All rights reserved. ;Distributed under a Creative Commons Attribution 4.0 International License ;2012 Elsevier Inc. All rights reserved. 2012 ;ISSN: 1097-2765 ;EISSN: 1097-4164 ;DOI: 10.1016/j.molcel.2012.08.018 ;PMID: 23000176

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