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EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols

Leukemia, 2012-09, Vol.26 (9), p.1986-2010 [Peer Reviewed Journal]

COPYRIGHT 2012 Nature Publishing Group ;Copyright Nature Publishing Group Sep 2012 ;Distributed under a Creative Commons Attribution 4.0 International License ;Copyright © 2012 Macmillan Publishers Limited 2012 Macmillan Publishers Limited ;ISSN: 0887-6924 ;EISSN: 1476-5551 ;DOI: 10.1038/leu.2012.122 ;PMID: 22948490

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Title:
EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols
Author: Kalina, T ; Flores-Montero, J ; van der Velden, V H J ; Martin-Ayuso, M ; Böttcher, S ; Ritgen, M ; Almeida, J ; Lhermitte, L ; Asnafi, V ; Mendonça, A ; de Tute, R ; Cullen, M ; Sedek, L ; Vidriales, M B ; Pérez, J J ; te Marvelde, J G ; Mejstrikova, E ; Hrusak, O ; Szczepański, T ; van Dongen, J J M ; Orfao, A
Subjects: Antibodies ; Antibodies, Monoclonal ; Biomarkers, Tumor - immunology ; Diagnosis ; Europe ; Flow cytometry ; Flow Cytometry - instrumentation ; Flow Cytometry - methods ; Flow Cytometry - standards ; Hematologic Neoplasms - diagnosis ; Hematologic Neoplasms - immunology ; Hematology ; Human health and pathology ; Humans ; Immunophenotyping - standards ; Laboratories - standards ; Leukemia ; Life Sciences ; Physiological aspects ; Prognosis ; Special Report ; Viral antibodies
Is Part Of: Leukemia, 2012-09, Vol.26 (9), p.1986-2010
Description: The EU-supported EuroFlow Consortium aimed at innovation and standardization of immunophenotyping for diagnosis and classification of hematological malignancies by introducing 8-color flow cytometry with fully standardized laboratory procedures and antibody panels in order to achieve maximally comparable results among different laboratories. This required the selection of optimal combinations of compatible fluorochromes and the design and evaluation of adequate standard operating procedures (SOPs) for instrument setup, fluorescence compensation and sample preparation. Additionally, we developed software tools for the evaluation of individual antibody reagents and antibody panels. Each section describes what has been evaluated experimentally versus adopted based on existing data and experience. Multicentric evaluation demonstrated high levels of reproducibility based on strict implementation of the EuroFlow SOPs and antibody panels. Overall, the 6 years of extensive collaborative experiments and the analysis of hundreds of cell samples of patients and healthy controls in the EuroFlow centers have provided for the first time laboratory protocols and software tools for fully standardized 8-color flow cytometric immunophenotyping of normal and malignant leukocytes in bone marrow and blood; this has yielded highly comparable data sets, which can be integrated in a single database.
Publisher: England: Nature Publishing Group
Language: English
Identifier: ISSN: 0887-6924
EISSN: 1476-5551
DOI: 10.1038/leu.2012.122
PMID: 22948490
Source: MEDLINE
ProQuest Central

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EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols

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