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Standardizing immunophenotyping for the Human Immunology Project

Nature reviews. Immunology, 2012-03, Vol.12 (3), p.191-200 [Peer Reviewed Journal]

COPYRIGHT 2012 Nature Publishing Group ;COPYRIGHT 2012 Nature Publishing Group ;Copyright Nature Publishing Group Mar 2012 ;2012 Macmillan Publishers Limited. All rights reserved 2012 ;ISSN: 1474-1733 ;EISSN: 1474-1741 ;DOI: 10.1038/nri3158 ;PMID: 22343568

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Title:
Standardizing immunophenotyping for the Human Immunology Project
Author: Maecker, Holden T ; McCoy, J Philip ; Nussenblatt, Robert
Subjects: Animals ; Data processing ; Flow cytometry ; Flow Cytometry - instrumentation ; Flow Cytometry - methods ; Flow Cytometry - standards ; Humans ; Immune system ; Immunophenotyping - instrumentation ; Immunophenotyping - methods ; Immunophenotyping - standards ; Leukocytes, Mononuclear - immunology ; Mice ; Physiological aspects ; Reference Standards ; Standardization ; T cells
Is Part Of: Nature reviews. Immunology, 2012-03, Vol.12 (3), p.191-200
Description: The heterogeneity in the healthy human immune system, and the immunological changes that portend various diseases, have been only partially described. Their comprehensive elucidation has been termed the 'Human Immunology Project'. The accurate measurement of variations in the human immune system requires precise and standardized assays to distinguish true biological changes from technical artefacts. Thus, to be successful, the Human Immunology Project will require standardized assays for immunophenotyping humans in health and disease. A major tool in this effort is flow cytometry, which remains highly variable with regard to sample handling, reagents, instrument setup and data analysis. In this Review, we outline the current state of standardization of flow cytometry assays and summarize the steps that are required to enable the Human Immunology Project.
Publisher: England: Nature Publishing Group
Language: English
Identifier: ISSN: 1474-1733
EISSN: 1474-1741
DOI: 10.1038/nri3158
PMID: 22343568
Source: MEDLINE
Alma/SFX Local Collection
ProQuest Central

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Standardizing immunophenotyping for the Human Immunology Project

COPYRIGHT 2012 Nature Publishing Group ;COPYRIGHT 2012 Nature Publishing Group ;Copyright Nature Publishing Group Mar 2012 ;2012 Macmillan Publishers Limited. All rights reserved 2012 ;ISSN: 1474-1733 ;EISSN: 1474-1741 ;DOI: 10.1038/nri3158 ;PMID: 22343568

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