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Mechanical stretch and shear flow induced reorganization and recruitment of fibronectin in fibroblasts

Scientific reports, 2011-11, Vol.1 (1), p.147-147, Article 147 [Peer Reviewed Journal]

Copyright Nature Publishing Group Nov 2011 ;Copyright © 2011, Macmillan Publishers Limited. All rights reserved 2011 Macmillan Publishers Limited. All rights reserved ;ISSN: 2045-2322 ;EISSN: 2045-2322 ;DOI: 10.1038/srep00147 ;PMID: 22355663

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Title:
Mechanical stretch and shear flow induced reorganization and recruitment of fibronectin in fibroblasts
Author: Steward, Jr, Robert L ; Cheng, Chao-Min ; Ye, Jonathan D ; Bellin, Robert M ; LeDuc, Philip R
Subjects: Animals ; Biomechanical Phenomena ; Fibrils ; Fibroblasts ; Fibroblasts - physiology ; Fibroblasts - ultrastructure ; Fibronectin ; Fibronectins - chemistry ; Fibronectins - physiology ; Fibronectins - ultrastructure ; Fluid flow ; Image Processing, Computer-Assisted ; Localization ; Mechanical stimuli ; Mice ; Microscopy, Confocal ; Microscopy, Fluorescence ; NIH 3T3 Cells ; Recruitment ; Rheology ; rho GTP-Binding Proteins - antagonists & inhibitors ; Shear Strength ; Shear stress ; Stress, Mechanical
Is Part Of: Scientific reports, 2011-11, Vol.1 (1), p.147-147, Article 147
Description: It was our objective to study the role of mechanical stimulation on fibronectin (FN) reorganization and recruitment by exposing fibroblasts to shear fluid flow and equibiaxial stretch. Mechanical stimulation was also combined with a Rho inhibitor to probe their coupled effects on FN. Mechanically stimulated cells revealed a localization of FN around the cell periphery as well as an increase in FN fibril formation. Mechanical stimulation coupled with chemical stimulation also revealed an increase in FN fibrils around the cell periphery. Complimentary to this, fibroblasts exposed to fluid shear stress structurally rearranged pre-coated surface FN, but unstimulated and stretched cells did not. These results show that mechanical stimulation directly affected FN reorganization and recruitment, despite perturbation by chemical stimulation. Our findings will help elucidate the mechanisms of FN biosynthesis and organization by furthering the link of the role of mechanics with FN.
Publisher: England: Nature Publishing Group
Language: English
Identifier: ISSN: 2045-2322
EISSN: 2045-2322
DOI: 10.1038/srep00147
PMID: 22355663
Source: MEDLINE
PubMed Central
ProQuest Central
DOAJ Directory of Open Access Journals

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Mechanical stretch and shear flow induced reorganization and recruitment of fibronectin in fibroblasts

Copyright Nature Publishing Group Nov 2011 ;Copyright © 2011, Macmillan Publishers Limited. All rights reserved 2011 Macmillan Publishers Limited. All rights reserved ;ISSN: 2045-2322 ;EISSN: 2045-2322 ;DOI: 10.1038/srep00147 ;PMID: 22355663

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