Language:

Interaction of CREB and PGC-1α Induces Fibronectin Type III Domain-Containing Protein 5 Expression in C2C12 Myotubes

Cellular physiology and biochemistry, 2018-01, Vol.50 (4), p.1574-1584 [Peer Reviewed Journal]

2018 The Author(s). Published by S. Karger AG, Basel ;2018 The Author(s). Published by S. Karger AG, Basel. ;ISSN: 1015-8987 ;EISSN: 1421-9778 ;DOI: 10.1159/000494655 ;PMID: 30359971

Full text available

Citations Cited by

Actions
1. Add to My Research
2. Remove from My Research
3. E-mail
4. Print
5. Permalink
6. Citation
7. EasyBib
8. EndNote
9. RefWorks
10. Delicious
11. Export RIS
12. Export BibTeX

Title:
Interaction of CREB and PGC-1α Induces Fibronectin Type III Domain-Containing Protein 5 Expression in C2C12 Myotubes
Author: Yang, Xiu-ying ; Tse, Margaret C.L. ; Hu, Xiang ; Jia, Wei-hua ; Du, Guan-hua ; Chan, Chi Bun
Subjects: Adenosine Triphosphate - metabolism ; Animals ; Biosynthesis ; C2C12 ; Cell Line ; Colforsin - pharmacology ; Cyclic AMP - metabolism ; Cyclic AMP Response Element-Binding Protein - genetics ; Cyclic AMP Response Element-Binding Protein - metabolism ; Diabetes ; Exercise ; Fatty acids ; Fibronectins - genetics ; Fibronectins - metabolism ; Fibronectins - pharmacology ; FNDC5 ; Glucose ; HEK293 Cells ; Homeostasis ; Humans ; Irisin ; Isoproterenol - pharmacology ; Kinases ; Metabolism ; Mice ; Muscle Fibers, Skeletal - cytology ; Muscle Fibers, Skeletal - drug effects ; Muscle Fibers, Skeletal - metabolism ; Musculoskeletal system ; Myokine ; Original Paper ; Oxidative Phosphorylation - drug effects ; Oxygen Consumption - drug effects ; Penicillin ; Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha - genetics ; Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha - metabolism ; PGC-1α ; Physical fitness ; Proteins ; Recombinant Proteins - biosynthesis ; Recombinant Proteins - pharmacology ; Respiration ; Skeletal muscle ; Statistical analysis
Is Part Of: Cellular physiology and biochemistry, 2018-01, Vol.50 (4), p.1574-1584
Description: Background/Aims: Fibronectin type III domain-containing protein 5 (FNDC5), also known as irisin, is a myokine secreted from muscle in response to exercise. However, the molecular mechanisms that regulate FNDC5 expression and the functional significance of irisn in skeletal muscle remain unknown. In this study, we explored the potential pathways that induce FNDC5 expression and delineated the metabolic effects of irisin on skeletal muscle. Methods: C2C12 myotubes were treated with drugs at various concentrations and durations. The expression and activation of genes were measured by real-time polymerase chain reaction (qRT-PCR) and Western blotting. Oxidative phosphorylation was quantified by measuring the oxygen consumption rate (OCR). Results: We found that the exercise-mimicking treatment (cAMP, forskolin and isoproterenol) increased Fndc5 expression in C2C12 myotubes. CREB over-expressed C2C12 myotubes displayed higher Fndc5 expression. CREB over-expression also promoted peroxisome proliferator-activated receptor gamma coactivator 1α (PGC-1α) expression. PGC-1α-induced Fndc5 expression was blocked when the dominant negative form of CREB (S133A) was present. PGC-1α mutation (S570A) also decreased Fndc5 expression. Immunoprecipitation showed that overexpressed PGC-1α complexed with CREB in HEK293 cells. C2C12 myotubes treated with forskolin also increased endogenous CREB and PGC-1α binding. Functionally, irisin treatment increased mitochondrial respiration, enhanced ATP production, promoted fatty acid oxidation but decreased glycolysis in myotubes. Conclusion: Our observation indicates that cAMP-mediated PGC-1α/CREB interaction triggers Fndc5 expression, which acts as an autocrine/paracrine to shape the metabolic phenotype of myotubes.
Publisher: Basel, Switzerland: S. Karger AG
Language: English
Identifier: ISSN: 1015-8987
EISSN: 1421-9778
DOI: 10.1159/000494655
PMID: 30359971
Source: MEDLINE
ProQuest Central
DOAJ Directory of Open Access Journals
Karger Open Access

Back to results list


INSPIRE LIBRARY - TON DUC THANG UNIVERSITY	(84-028) 37 755 057	Feedback
19 Nguyen Huu Tho St. Dist.7, HCM	thuvien@tdtu.edu.vn	Feedback

Interaction of CREB and PGC-1α Induces Fibronectin Type III Domain-Containing Protein 5 Expression in C2C12 Myotubes

2018 The Author(s). Published by S. Karger AG, Basel ;2018 The Author(s). Published by S. Karger AG, Basel. ;ISSN: 1015-8987 ;EISSN: 1421-9778 ;DOI: 10.1159/000494655 ;PMID: 30359971

Searching Remote Databases, Please Wait