skip to main content

Sign out
Sign in

Language:

VEGF(121)b, a new member of the VEGF(xxx)b family of VEGF-A splice isoforms, inhibits neovascularisation and tumour growth in vivo

British journal of cancer, 2009-10, Vol.101 (7), p.1183 [Peer Reviewed Journal]

EISSN: 1532-1827 ;DOI: 10.1038/sj.bjc.6605249 ;PMID: 19707198

Full text available

Citations Cited by

Actions
1. Add to My Research
2. Remove from My Research
3. E-mail
4. Print
5. Permalink
6. Citation
7. EasyBib
8. EndNote
9. RefWorks
10. Delicious
11. Export RIS
12. Export BibTeX

Title:
VEGF(121)b, a new member of the VEGF(xxx)b family of VEGF-A splice isoforms, inhibits neovascularisation and tumour growth in vivo
Author: Rennel, E S ; Varey, A H R ; Churchill, A J ; Wheatley, E R ; Stewart, L ; Mather, S ; Bates, D O ; Harper, S J
Subjects: Aged ; Aged, 80 and over ; Alternative Splicing ; Animals ; Apoptosis ; Cell Line, Tumor ; Cell Movement ; Colon - chemistry ; Colonic Neoplasms - chemistry ; Endothelial Cells - physiology ; Female ; Humans ; Male ; Mice ; Middle Aged ; Neoplasms - pathology ; Neoplasms - prevention & control ; Neovascularization, Pathologic - prevention & control ; Protein Isoforms ; Tissue Distribution ; Vascular Endothelial Growth Factor A - pharmacokinetics ; Vascular Endothelial Growth Factor A - physiology
Is Part Of: British journal of cancer, 2009-10, Vol.101 (7), p.1183
Description: The key mediator of new vessel formation in cancer and other diseases is VEGF-A. VEGF-A exists as alternatively spliced isoforms - the pro-angiogenic VEGF(xxx) family generated by exon 8 proximal splicing, and a sister family, termed VEGF(xxx)b, exemplified by VEGF(165)b, generated by distal splicing of exon 8. However, it is unknown whether this anti-angiogenic property of VEGF(165)b is a general property of the VEGF(xxx)b family of isoforms. The mRNA and protein expression of VEGF(121)b was studied in human tissue. The effect of VEGF(121)b was analysed by saturation binding to VEGF receptors, endothelial migration, apoptosis, xenograft tumour growth, pre-retinal neovascularisation and imaging of biodistribution in tumour-bearing mice with radioactive VEGF(121)b. The existence of VEGF(121)b was confirmed in normal human tissues. VEGF(121)b binds both VEGF receptors with similar affinity as other VEGF isoforms, but inhibits endothelial cell migration and is cytoprotective to endothelial cells through VEGFR-2 activation. Administration of VEGF(121)b normalised retinal vasculature by reducing both angiogenesis and ischaemia. VEGF(121)b reduced the growth of xenografted human colon tumours in association with reduced microvascular density, and an intravenous bolus of VEGF(121)b is taken up into colon tumour xenografts. Here we identify a second member of the family, VEGF(121)b, with similar properties to those of VEGF(165)b, and underline the importance of the six amino acids of exon 8b in the anti-angiogenic activity of the VEGF(xxx)b isoforms.
Publisher: England
Language: English
Identifier: EISSN: 1532-1827
DOI: 10.1038/sj.bjc.6605249
PMID: 19707198
Source: GFMER Free Medical Journals
MEDLINE
PubMed Central
ProQuest Central

Back to results list


	INSPIRE LIBRARY - TON DUC THANG UNIVERSITY		(84-028) 37 755 057	Feedback
	19 Nguyen Huu Tho St. Dist.7, HCM		thuvien@tdtu.edu.vn	Feedback

Copyright © 2017 INSPIRE LIBRARY - TON DUC THANG UNIVERSITY

Searching Remote Databases, Please Wait

Searching for
inscope:(TDTS),scope:(SFX),scope:(TDT),scope:(SEN),primo_central_multiple_fe
Show me what you have so far