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Topical calcitriol restores the impairment of epidermal permeability and antimicrobial barriers induced by corticosteroids

British journal of dermatology (1951), 2010-06, Vol.162 (6), p.1251-1260 [Peer Reviewed Journal]

2010 The Authors. Journal Compilation © 2010 British Association of Dermatologists ;2015 INIST-CNRS ;2010 The Authors. Journal Compilation © 2010 British Association of Dermatologists. ;ISSN: 0007-0963 ;EISSN: 1365-2133 ;DOI: 10.1111/j.1365-2133.2010.09760.x ;PMID: 20302580 ;CODEN: BJDEAZ

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Title:
Topical calcitriol restores the impairment of epidermal permeability and antimicrobial barriers induced by corticosteroids
Author: Hong, S.P. ; Oh, Y. ; Jung, M. ; Lee, S. ; Jeon, H. ; Cho, M-Y. ; Lee, S.H. ; Choi, E.H.
Subjects: Administration, Topical ; Animals ; Antimicrobial agents ; antimicrobial peptide ; Antimicrobial peptides ; Biological and medical sciences ; Blotting, Western ; Calcitriol ; Calcitriol - pharmacology ; Calcitriol - therapeutic use ; Calcium Channel Agonists - pharmacology ; Calcium Channel Agonists - therapeutic use ; Clobetasol - analogs & derivatives ; Clobetasol - pharmacology ; Corticoids ; corticosteroids ; Dermatology ; Disease Models, Animal ; Electron microscopy ; Enzymes ; Enzymes - metabolism ; epidermal permeability barrier ; Epidermis ; Epidermis - drug effects ; Epidermis - metabolism ; Epidermis - pathology ; Fatty-acid synthase ; Female ; Fluorescence ; Glucocorticoids - pharmacology ; Hairless ; Immunohistochemistry ; Lipids ; Medical sciences ; Mice ; Mice, Hairless ; Microscopy, Electron ; Oxazines - administration & dosage ; Permeability ; Permeability - drug effects ; Polymerase chain reaction ; Polymerase Chain Reaction - methods ; Propionic acid ; Psoriasis ; Skin Absorption - drug effects ; Stratum corneum ; Up-Regulation ; Vitamin D ; Vitamin D3 ; Western blotting
Is Part Of: British journal of dermatology (1951), 2010-06, Vol.162 (6), p.1251-1260
Description: Summary Background The active form of vitamin D3, calcitriol, is widely used for the treatment of psoriasis, with or without topical corticosteroids. Topical corticosteroids are known to disrupt permeability and antimicrobial barriers, even with short‐term use. Yet, the effect of topical calcitriol on epidermal permeability and antimicrobial barriers disrupted by topical corticosteroids has not been determined. Objectives To examine the effect of calcitriol on epidermal permeability and antimicrobial barrier function that has been impaired by corticosteroids, as well as to elucidate the mechanism of improvement. Material and methods Topical calcitriol or the control vehicle was applied to each flank of hairless mice 20 min after treatment with topical clobetasol propionate and repeated every 12 h for 3·5 days. Barrier function assessment, Nile red staining, electron microscopy, immunohistochemistry, Western blotting, and real‐time reverse transcriptase–polymerase chain reaction studies were performed 24 h after the last application. Results Epidermis co‐treated with topical calcitriol showed an improvement of stratum corneum integrity and barrier recovery, more intense fluorescence staining with Nile red, and an increase in lamellar body (LB) maturation and density, as well as upregulation of major epidermal lipid synthesis‐related enzymes (3‐hydroxy‐3‐methylglutaryl‐CoA, serine‐palmitoyl transferase and fatty acid synthase), mouse beta‐defensin 3, cathelin‐related antimicrobial peptide and vitamin D receptor. Conclusions We found that topical calcitriol restored both the epidermal permeability and antimicrobial barrier that had been impaired by corticosteroids. This restoration was mediated by both an activation of the cutaneous vitamin D pathway and an increase of epidermal lipids and antimicrobial peptides, promoted by the formation of the LB and the activity of epidermal lipid synthesis‐related enzymes.
Publisher: Oxford, UK: Blackwell Publishing Ltd
Language: English
Identifier: ISSN: 0007-0963
EISSN: 1365-2133
DOI: 10.1111/j.1365-2133.2010.09760.x
PMID: 20302580
CODEN: BJDEAZ
Source: MEDLINE
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Topical calcitriol restores the impairment of epidermal permeability and antimicrobial barriers induced by corticosteroids

2010 The Authors. Journal Compilation © 2010 British Association of Dermatologists ;2015 INIST-CNRS ;2010 The Authors. Journal Compilation © 2010 British Association of Dermatologists. ;ISSN: 0007-0963 ;EISSN: 1365-2133 ;DOI: 10.1111/j.1365-2133.2010.09760.x ;PMID: 20302580 ;CODEN: BJDEAZ

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