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Evaluation of human sperm-zona pellucida tight binding by presence of monoclonal antibodies to sperm antigens

Journal of reproductive immunology, 1991-04, Vol.19 (3), p.269-285 [Peer Reviewed Journal]

1991 ;1991 INIST-CNRS ;1992 INIST-CNRS ;ISSN: 0165-0378 ;EISSN: 1872-7603 ;DOI: 10.1016/0165-0378(91)90040-W ;PMID: 1865391 ;CODEN: JRIMDR

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Title:
Evaluation of human sperm-zona pellucida tight binding by presence of monoclonal antibodies to sperm antigens
Author: Mahony, Mary Condon ; Fulgham, David L. ; Blackmore, Peter F. ; Alexander, Nancy J.
Subjects: Antibodies, Monoclonal - immunology ; Antibodies, Monoclonal - pharmacology ; Antigens - immunology ; Biological and medical sciences ; Birth control ; Calcium - metabolism ; calcium influx ; capacitation ; Female ; fertilization ; Fundamental and applied biological sciences. Psychology ; Gynecology. Andrology. Obstetrics ; Humans ; Male ; Medical sciences ; monoclonal antibodies ; Population ; sperm antigen ; Sperm Capacitation ; Sperm Motility ; Sperm-Ovum Interactions ; Spermatozoa - immunology ; Spermatozoa - metabolism ; Sterility. Assisted procreation ; Vertebrates: reproduction ; zona pellucida ; Zona Pellucida - metabolism
Is Part Of: Journal of reproductive immunology, 1991-04, Vol.19 (3), p.269-285
Description: Characterized WHO monoclonal antibodies (MAbs) to human sperm antigens were evaluated as to whether they inhibited sperm-zona pellucida tight binding as assessed by the hemizona assay (HZA). Of the 26 MAbs tested, only one inhibited zona binding. The whole sperm-specific MAb inhibited zona binding by 70%. The MAb also caused strong agglutination. Two procedures, Sephadex column chromatography and papain digestion, were used to determine whether agglutination or steric hindrance was a factor in the capability of MAb to inhibit zona binding. However, inhibition remained comparable to previous results. The MAb did not prevent capacitation, nor calcium influx and the resulting increase in hyperactivated motility and acrosome reaction. Since its inhibitory influence is not due to agglutination factors, steric hindrance or prevention of normal pre-fertilization maturation, the MAb may be blocking a portion of the zona binding receptor and may be useful in elucidating sperm antigens important to sperm-egg interaction. The approach used in this study allows definition of sperm surface antigens involved in zona pellucida binding.
Publisher: Shannon: Elsevier Ireland Ltd
Language: English
Identifier: ISSN: 0165-0378
EISSN: 1872-7603
DOI: 10.1016/0165-0378(91)90040-W
PMID: 1865391
CODEN: JRIMDR
Source: MEDLINE
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Evaluation of human sperm-zona pellucida tight binding by presence of monoclonal antibodies to sperm antigens

1991 ;1991 INIST-CNRS ;1992 INIST-CNRS ;ISSN: 0165-0378 ;EISSN: 1872-7603 ;DOI: 10.1016/0165-0378(91)90040-W ;PMID: 1865391 ;CODEN: JRIMDR

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