Language:

ROR2 deficit may induce the tetralogy of Fallot via down-regulating of β-catenin/SOX3/HSPA6 in vitro and in vivo

Journal of cellular and molecular medicine, 2023-11, Vol.27 (22), p.3539-3552 [Peer Reviewed Journal]

2023 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. ;2023. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. ;ISSN: 1582-1838 ;EISSN: 1582-4934 ;DOI: 10.1111/jcmm.17969 ;PMID: 37749917

Full text available

Citations Cited by

Actions
1. Add to My Research
2. Remove from My Research
3. E-mail
4. Print
5. Permalink
6. Citation
7. EasyBib
8. EndNote
9. RefWorks
10. Delicious
11. Export RIS
12. Export BibTeX

Title:
ROR2 deficit may induce the tetralogy of Fallot via down-regulating of β-catenin/SOX3/HSPA6 in vitro and in vivo
Author: Guo, Rui ; Guo, Yiran Zhou ; Zhou, Qing ; Li, Guoju ; Du, Zhanghui ; Shi, Yefei ; Xing, Quansheng
Subjects: Antibodies ; Antigens ; Apoptosis ; beta Catenin - genetics ; beta Catenin - metabolism ; Cardiovascular disease ; Cell cycle ; Cell proliferation ; Cell viability ; Cholecystokinin ; Congenital diseases ; Down-regulation ; Down-Regulation - genetics ; Experiments ; Flow cytometry ; Heart ; Heart diseases ; Heat shock ; Humans ; Kinases ; Ligands ; Medical research ; Protein-tyrosine kinase receptors ; Proteins ; Pulmonary arteries ; Reagents ; Receptor Tyrosine Kinase-like Orphan Receptors - genetics ; S phase ; Signal transduction ; SOXB1 Transcription Factors - metabolism ; Tetralogy of Fallot ; Tetralogy of Fallot - genetics ; Transcription factors ; Transcriptomes ; Wnt protein ; Wnt Signaling Pathway - genetics ; β-Catenin
Is Part Of: Journal of cellular and molecular medicine, 2023-11, Vol.27 (22), p.3539-3552
Description: Tetralogy of Fallot (TOF) is the highly conventional appearance of cyanotic congenital heart disease. Our study aimed to assess the involvement of receptor tyrosine kinase-like orphan receptor 2 (ROR2) in TOF and elucidate the specific mechanism. Upon investigation of human tissue samples, we observed a decrease in ROR2 expression in TOF patients compared to healthy control individuals. Transcriptome analysis revealed diminished ROR2 expression in TOF pathological samples relative to normal tissues. Of the 2246 genes that exhibited altered expression, 886 were upregulated, while 1360 were down-regulated. KEGG analysis and GO analysis of the differentially expressed genes indicated that these genes were significantly enriched in the Wnt signalling pathway, apoptosis and cardiac development function. Importantly, ROR2 was the only gene shared among the three pathways. Furthermore, interference with ROR2 promotes apoptosis and curtails cell proliferation in vitro. The knockdown of the ROR2 gene in AC16 cells resulted in a significant decrease in Edu-positive cells. Flow cytometry studies indicated an increase in the percentage of cells in the S phase. In contrast, the G2/M cell cycle transition was blocked in the ROR2-knockdown group, leading to a significant increase in apoptosis. Moreover, the CCK-8 cell viability assay demonstrated a reduced proliferation in the ROR2-knockdown group. Furthermore, both in vivo and in vitro data indicated that the expression of HSPA6 (Recombinant Heat Shock 70 kDa Protein6), an essential gene enriched in cardiac tissue and associated with apoptosis, was down-regulated following ROR2 knockdown mediated by the β-catenin/SOX3 signalling pathway. In conclusion, low expression of ROR2 plays a crucial role in the occurrence and development of TOF, which may be related to the downregulation of HSPA6 through the β-catenin/SOX3 signalling pathway.
Publisher: England: John Wiley & Sons, Inc
Language: English
Identifier: ISSN: 1582-1838
EISSN: 1582-4934
DOI: 10.1111/jcmm.17969
PMID: 37749917
Source: PubMed
Journals@Ovid Open Access Journal Collection Rolling
MEDLINE
Wiley Online Library Open Access
ProQuest Central
DOAJ Directory of Open Access Journals

Back to results list

Previous Result 14 Next Go to Next page


INSPIRE LIBRARY - TON DUC THANG UNIVERSITY	(84-028) 37 755 057	Feedback
19 Nguyen Huu Tho St. Dist.7, HCM	thuvien@tdtu.edu.vn	Feedback

ROR2 deficit may induce the tetralogy of Fallot via down-regulating of β-catenin/SOX3/HSPA6 in vitro and in vivo

Searching Remote Databases, Please Wait