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A 163‐bp insertion in the Capana10g000198 encoding a MYB transcription factor causes male sterility in pepper (Capsicum annuum L.)

The Plant journal : for cell and molecular biology, 2023-02, Vol.113 (3), p.521-535 [Peer Reviewed Journal]

2022 Society for Experimental Biology and John Wiley & Sons Ltd. ;Copyright © 2023 Society for Experimental Biology and John Wiley & Sons Ltd ;ISSN: 0960-7412 ;EISSN: 1365-313X ;DOI: 10.1111/tpj.16064 ;PMID: 36534067

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Title:
A 163‐bp insertion in the Capana10g000198 encoding a MYB transcription factor causes male sterility in pepper (Capsicum annuum L.)
Author: Dong, Jichi ; Hu, Fang ; Guan, Wendong ; Yuan, Fanchong ; Lai, Zepei ; Zhong, Jian ; Liu, Jia ; Wu, Zhiming ; Cheng, Jiaowen ; Hu, Kailin
Subjects: Anthers ; Capsicum - genetics ; Capsicum - physiology ; Capsicum annuum ; Capsicum annuum L ; Degeneration ; Exploitation ; fine mapping ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation, Plant - genetics ; Gene silencing ; Genes ; genic male sterility ; Heterosis ; Insertion ; Linkage analysis ; Male ; Male sterility ; Males ; msc‐3 ; Mutants ; MYB transcription factor ; Peppers ; Phenotypes ; Plant Infertility - genetics ; Pollen ; Regulatory mechanisms (biology) ; solo LTR insertion ; Tetrads ; Transcription factors ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Transcriptomes ; Vegetables ; Viruses
Is Part Of: The Plant journal : for cell and molecular biology, 2023-02, Vol.113 (3), p.521-535
Description: SUMMARY Male sterility provides an efficient approach for commercial exploitation of heterosis. Despite more than 20 genic male sterile (GMS) mutants documented in pepper (Capsicum annuum L.), only two causal genes have been successfully identified. Here, a novel spontaneous recessive GMS mutant, designated msc‐3, is identified and characterized at both phenotypic and histological levels. Pollen abortion of msc‐3 mutant may be due to the delayed tapetum degradation, leading to the non‐degeneration of tetrads callosic wall. Then, a modified MutMap method and molecular marker linkage analysis were employed to fine mapping the msc‐3 locus, which was delimited to the ~139.91‐kb region harboring 10 annotated genes. Gene expression and structure variation analyses indicate the Capana10g000198, encoding a R2R3‐MYB transcription factor, is the best candidate gene for the msc‐3 locus. Expression profiling analysis shows the Capana10g000198 is an anther‐specific gene, and a 163‐bp insertion in the Capana10g000198 is highly correlated with the male sterile (MS) phenotype. Additionally, downregulation of Capana10g000198 in male fertile plants through virus‐induced gene silencing resulted in male sterility. Finally, possible regulatory relationships of the msc‐3 gene with the other two reported pepper GMS genes, msc‐1 and msc‐2, have been studied, and comparative transcriptome analysis reveals the expression of 16 GMS homologs are significantly downregulated in the MS anthers. Overall, our results reveal that Capana10g000198 is the causal gene underlying the msc‐3 locus, providing important theoretical clues and basis for further in‐depth study on the regulatory mechanisms of pollen development in pepper. Significance Statement In this study, a novel pepper genic male sterile mutant and the causal gene were identified, and the regulatory mechanism for complex fertility development was preliminarily studied. Our findings provide important theoretical clues and basis for further in‐depth study on the regulatory mechanisms of pollen development in pepper.
Publisher: England: Blackwell Publishing Ltd
Language: English
Identifier: ISSN: 0960-7412
EISSN: 1365-313X
DOI: 10.1111/tpj.16064
PMID: 36534067
Source: IngentaConnect Open Access
MEDLINE

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A 163‐bp insertion in the Capana10g000198 encoding a MYB transcription factor causes male sterility in pepper (Capsicum annuum L.)

2022 Society for Experimental Biology and John Wiley & Sons Ltd. ;Copyright © 2023 Society for Experimental Biology and John Wiley & Sons Ltd ;ISSN: 0960-7412 ;EISSN: 1365-313X ;DOI: 10.1111/tpj.16064 ;PMID: 36534067

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