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Faster, sharper, and deeper: structured illumination microscopy for biological imaging

Nature methods, 2018-12, Vol.15 (12), p.1011-1019 [Peer Reviewed Journal]

COPYRIGHT 2018 Nature Publishing Group ;Copyright Nature Publishing Group Dec 2018 ;ISSN: 1548-7091 ;EISSN: 1548-7105 ;DOI: 10.1038/s41592-018-0211-z ;PMID: 30478322

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  • Title:
    Faster, sharper, and deeper: structured illumination microscopy for biological imaging
  • Author: Wu, Yicong ; Shroff, Hari
  • Subjects: Fluorescence microscopy ; Illumination ; Image resolution ; Medical research ; Microscopy ; Mitochondria
  • Is Part Of: Nature methods, 2018-12, Vol.15 (12), p.1011-1019
  • Description: Structured illumination microscopy (SIM) allows rapid, super-resolution (SR) imaging in live specimens. We review recent technical advances in SR-SIM, with emphasis on imaging speed, resolution, and depth. Since its introduction decades ago, the technique has grown to offer myriad implementations, each with its own strengths and weaknesses. We discuss these, aiming to provide a practical guide for biologists and to highlight which approach is best suited to a given application.
  • Publisher: United States: Nature Publishing Group
  • Language: English
  • Identifier: ISSN: 1548-7091
    EISSN: 1548-7105
    DOI: 10.1038/s41592-018-0211-z
    PMID: 30478322
  • Source: ProQuest Central
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