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Development of a paper-based lateral flow immunoassay for simultaneous detection of lipopolysaccharides of Salmonella serovars

Analytical and bioanalytical chemistry, 2018-01, Vol.410 (3), p.863-868 [Peer Reviewed Journal]

Springer-Verlag GmbH Germany 2017 ;COPYRIGHT 2018 Springer ;Springer-Verlag GmbH Germany 2017. ;ISSN: 1618-2642 ;EISSN: 1618-2650 ;DOI: 10.1007/s00216-017-0643-9 ;PMID: 28971240

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Title:
Development of a paper-based lateral flow immunoassay for simultaneous detection of lipopolysaccharides of Salmonella serovars
Author: Schenk, Florian ; Weber, Patricia ; Vogler, Julian ; Hecht, Lars ; Dietzel, Andreas ; Gauglitz, Günter
Subjects: ABCs 16th Anniversary ; Analytical Chemistry ; Antibodies ; Biochemistry ; Calibration ; Cellulose esters ; Cellulose nitrate ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Colorimetry ; Diagnosis ; Epidemics ; Femtosecond ; Food Science ; Health aspects ; Identification and classification ; Image processing ; Immunoassay ; Laboratory Medicine ; Laser ablation ; Lipopolysaccharides ; Monitoring/Environmental Analysis ; Nanoparticles ; Porous materials ; Rapid Communication ; Salmonella ; Salmonella enteritidis
Is Part Of: Analytical and bioanalytical chemistry, 2018-01, Vol.410 (3), p.863-868
Description: Lateral flow type detection is becoming interesting not only in regions with a poor medical infrastructure but also for practitioners in day-to-day clinical work or for veterinary control in case of possible epidemics. In this work, we describe the first steps of development of a multi-channel strip with potential internal calibration of multiparametric and colorimetric lateral flow assays for the simultaneous detection of the lipopolysaccharides (LPS) of Salmonella typhimurium ( S . typhimurium ) and Salmonella enteritidis ( S . enteritidis ). We structured four channels in the nitrocellulose membrane with a Yb:KGW solid-state femtosecond laser (“cold” ablation process) to form distinct tracks of porous material and used gold nanoparticles for the labeling of the antibodies. In addition, calibration curves of the spot intensities of both serovars are presented, and it was shown that no cross reactivity between the different capture antibodies and LPS occurred. Finally, we detected LPS of both Salmonella serovars simultaneously. The color changes (spot intensities of the reaction zones) were evaluated using the open-source image-processing program ImageJ . Graphical abstract Multiparametric testing, strip A was tested with LPS S. enteritidis ( c=0.01 g/L) and LPS S.typhimurium ( c=0.0001 g/L), strip B with LPS S. enteritidis ( c=0.001 g/L) and LPS S. typhimurium ( c=0.001g/L) and strip C with LPS S. enteritidis (c=0.0001 g/L) and LPS S. typhimurium ( c=0.01 g/L), and read-out
Publisher: Berlin/Heidelberg: Springer Berlin Heidelberg
Language: English
Identifier: ISSN: 1618-2642
EISSN: 1618-2650
DOI: 10.1007/s00216-017-0643-9
PMID: 28971240
Source: ProQuest Central

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Development of a paper-based lateral flow immunoassay for simultaneous detection of lipopolysaccharides of Salmonella serovars

Springer-Verlag GmbH Germany 2017 ;COPYRIGHT 2018 Springer ;Springer-Verlag GmbH Germany 2017. ;ISSN: 1618-2642 ;EISSN: 1618-2650 ;DOI: 10.1007/s00216-017-0643-9 ;PMID: 28971240

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