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FgLPMO9A from Fusarium graminearum cleaves xyloglucan independently of the backbone substitution pattern

FEBS letters, 2016-10, Vol.590 (19), p.3346-3356 [Peer Reviewed Journal]

2016 Federation of European Biochemical Societies ;2016 Federation of European Biochemical Societies. ;ISSN: 0014-5793 ;EISSN: 1873-3468 ;DOI: 10.1002/1873-3468.12385 ;PMID: 27587308

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Title:
FgLPMO9A from Fusarium graminearum cleaves xyloglucan independently of the backbone substitution pattern
Author: Nekiunaite, Laura ; Petrović, Dejan M. ; Westereng, Bjørge ; Vaaje‐Kolstad, Gustav ; Hachem, Maher Abou ; Várnai, Anikó ; Eijsink, Vincent G.H.
Subjects: AA9 ; cellulose ; Cellulose - metabolism ; Fungal Proteins - chemistry ; Fungal Proteins - metabolism ; Fusarium - enzymology ; Fusarium graminearum ; Glucans - metabolism ; lytic polysaccharide monooxygenase ; Mixed Function Oxygenases - chemistry ; Mixed Function Oxygenases - metabolism ; Substrate Specificity ; Xylans - metabolism ; xyloglucan
Is Part Of: FEBS letters, 2016-10, Vol.590 (19), p.3346-3356
Description: Lytic polysaccharide monooxygenases (LPMOs) are important for the enzymatic conversion of biomass and seem to play a key role in degradation of the plant cell wall. In this study, we characterize an LPMO from the fungal plant pathogen Fusarium graminearum (FgLPMO9A) that catalyzes the mixed C1/C4 oxidative cleavage of cellulose and xyloglucan, but is inactive toward other (1,4)‐linked β‐glucans. Our findings indicate that FgLPMO9A has unprecedented broad specificity on xyloglucan, cleaving any glycosidic bond in the β‐glucan main chain, regardless of xylosyl substitutions. Interestingly, we found that when incubated with a mixture of xyloglucan and cellulose, FgLPMO9A efficiently attacks the xyloglucan, whereas cellulose conversion is inhibited. This suggests that removal of hemicellulose may be the true function of this LPMO during biomass conversion.
Publisher: England
Language: English
Identifier: ISSN: 0014-5793
EISSN: 1873-3468
DOI: 10.1002/1873-3468.12385
PMID: 27587308
Source: Geneva Foundation Free Medical Journals at publisher websites
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FgLPMO9A from Fusarium graminearum cleaves xyloglucan independently of the backbone substitution pattern

2016 Federation of European Biochemical Societies ;2016 Federation of European Biochemical Societies. ;ISSN: 0014-5793 ;EISSN: 1873-3468 ;DOI: 10.1002/1873-3468.12385 ;PMID: 27587308

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