skip to main content
Language:
Search Limited to: Search Limited to: Resource type Show Results with: Show Results with: Search type Index

The C-terminus of DSX super(F5) protein acts as a novel regulatory domain in Bombyx mori

Transgenic research, 2016-08, Vol.25 (4), p.491-497 [Peer Reviewed Journal]

ISSN: 0962-8819 ;EISSN: 1573-9368 ;DOI: 10.1007/s11248-016-9946-4

Full text available

Citations Cited by
  • Title:
    The C-terminus of DSX super(F5) protein acts as a novel regulatory domain in Bombyx mori
  • Author: Duan, Jianping ; Meng, Xianxin ; Ma, Sanyuan ; Wang, Feng ; Guo, Huozhen ; Zhang, Liying ; Zhao, Ping ; Kan, Yunchao ; Yao, Lunguang ; Xia, Qingyou
  • Subjects: Bombyx mori
  • Is Part Of: Transgenic research, 2016-08, Vol.25 (4), p.491-497
  • Description: The doublesex gene regulates the somatic sexual development of Bombyx mori by alternatively splicing into sex-specific splice forms. In our previous study, the splice form Bmdsx F7 , which encodes the BmDSX super(F5) protein, was found to be expressed in a female-specific manner and to contain a novel C-terminus. In this study, we aimed to investigate the role of this C-terminus. Two transgenic lines, L1 and L2, were constructed to ectopically express Bmdsx F7 in males. Phenotype and W chromosome-specific polymerase chain reaction (PCR) analysis showed that developmental abnormalities and sex reversal did not occur. Moreover, the sex ratio was also normal. Quantitative PCR revealed that the expression levels of SP1 and Vg were upregulated in the fat body of transgenic males. Additionally, the expression level of PBP was downregulated in the antenna of transgenic males. The results suggested that the C-terminus of BmDSX super(F5) functioned as a regulatory domain during regulation of downstream target gene expression and that BmDSX super(F5) participated in the sexual development of somatic cells together with other DSX proteins in B. mori.
  • Language: English
  • Identifier: ISSN: 0962-8819
    EISSN: 1573-9368
    DOI: 10.1007/s11248-016-9946-4
  • Source: ProQuest Central
Back to results list
INSPIRE LIBRARY - TON DUC THANG UNIVERSITY (84-028) 37 755 057 Feedback
19 Nguyen Huu Tho St. Dist.7, HCM thuvien@tdtu.edu.vn

Copyright © 2017 INSPIRE LIBRARY - TON DUC THANG UNIVERSITY

Searching Remote Databases, Please Wait