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The NLRP3 inflammasome is up-regulated in cardiac fibroblasts and mediates myocardial ischaemia-reperfusion injury

Cardiovascular research, 2013-07, Vol.99 (1), p.164-174 [Peer Reviewed Journal]

ISSN: 0008-6363 ;EISSN: 1755-3245 ;DOI: 10.1093/cvr/cvt091 ;PMID: 23580606

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Title:
The NLRP3 inflammasome is up-regulated in cardiac fibroblasts and mediates myocardial ischaemia-reperfusion injury
Author: Sandanger, Øystein ; Ranheim, Trine ; Vinge, Leif Erik ; Bliksøen, Marte ; Alfsnes, Katrine ; Finsen, Alexandra V ; Dahl, Christen P ; Askevold, Erik T ; Florholmen, Geir ; Christensen, Geir ; Fitzgerald, Katherine A ; Lien, Egil ; Valen, Guro ; Espevik, Terje ; Aukrust, Pål ; Yndestad, Arne
Subjects: Adenosine Triphosphate - metabolism ; Animals ; Apoptosis ; Apoptosis Regulatory Proteins ; CARD Signaling Adaptor Proteins ; Carrier Proteins - genetics ; Carrier Proteins - metabolism ; Caspase 1 - metabolism ; Cells, Cultured ; Cytoskeletal Proteins - deficiency ; Cytoskeletal Proteins - genetics ; Disease Models, Animal ; Fibroblasts - drug effects ; Fibroblasts - immunology ; Fibroblasts - metabolism ; Heart Ventricles - drug effects ; Heart Ventricles - immunology ; Heart Ventricles - metabolism ; Heart Ventricles - pathology ; Heart Ventricles - physiopathology ; Inflammasomes - genetics ; Inflammasomes - metabolism ; Interleukin-18 - genetics ; Interleukin-18 - metabolism ; Interleukin-1beta - genetics ; Interleukin-1beta - metabolism ; Lipopolysaccharides - pharmacology ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Myocardial Contraction ; Myocardial Infarction - genetics ; Myocardial Infarction - immunology ; Myocardial Infarction - metabolism ; Myocardial Infarction - pathology ; Myocardial Infarction - physiopathology ; Myocardial Reperfusion Injury - genetics ; Myocardial Reperfusion Injury - immunology ; Myocardial Reperfusion Injury - metabolism ; Myocardial Reperfusion Injury - pathology ; Myocardial Reperfusion Injury - physiopathology ; Myocardial Reperfusion Injury - prevention & control ; NF-kappa B - metabolism ; NLR Family, Pyrin Domain-Containing 3 Protein ; Potassium - metabolism ; Rats ; Rats, Wistar ; Receptors, Cytoplasmic and Nuclear - genetics ; Receptors, Cytoplasmic and Nuclear - metabolism ; RNA, Messenger - metabolism ; Time Factors ; Toll-Like Receptors - metabolism ; Up-Regulation ; Ventricular Function, Left
Is Part Of: Cardiovascular research, 2013-07, Vol.99 (1), p.164-174
Description: Nucleotide-binding oligomerization domain-Like Receptor with a Pyrin domain 3 (NLRP3) is considered necessary for initiating a profound sterile inflammatory response. NLRP3 forms multi-protein complexes with Apoptosis-associated Speck-like protein containing a Caspase recruitment domain (ASC) and Caspase-1, which activate pro-interleukin-1β (IL-1β) and pro-IL-18. The role of NLRP3 in cardiac cells is not known. Thus, we investigated the expression and function of NLRP3 during myocardial ischaemia. Myocardial infarction (MI) was induced in adult C57BL/6 mice and Wistar rats by ligation of the coronary artery. A marked increase in NLRP3, IL-1β, and IL-18 mRNA expression was found in the left ventricle after MI, primarily located to myocardial fibroblasts. In vitro studies in cells from adult mice showed that myocardial fibroblasts released IL-1β and IL-18 when primed with lipopolysaccharide and subsequently exposed to the danger signal adenosine triphosphate, a molecule released after tissue damage during MI. When hearts were isolated from NLRP3-deficient mice, perfused and subjected to global ischaemia and reperfusion, a marked improvement of cardiac function and reduction of hypoxic damage was found compared with wild-type hearts. This was not observed in ASC-deficient hearts, potentially reflecting a protective role of other ASC-dependent inflammasomes or inflammasome-independent effects of NLRP3. This study shows that the NLRP3 inflammasome is up-regulated in myocardial fibroblasts post-MI, and may be a significant contributor to infarct size development during ischaemia-reperfusion.
Publisher: England
Language: English
Identifier: ISSN: 0008-6363
EISSN: 1755-3245
DOI: 10.1093/cvr/cvt091
PMID: 23580606
Source: Geneva Foundation Free Medical Journals at publisher websites
MEDLINE
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The NLRP3 inflammasome is up-regulated in cardiac fibroblasts and mediates myocardial ischaemia-reperfusion injury

ISSN: 0008-6363 ;EISSN: 1755-3245 ;DOI: 10.1093/cvr/cvt091 ;PMID: 23580606

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