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TLR-induced NF-κB activation regulates NLRP3 expression in murine macrophages

FEBS letters, 2012-04, Vol.586 (7), p.1022-1026 [Peer Reviewed Journal]

2012 Federation of European Biochemical Societies ;FEBS Letters 586 (2012) 1873-3468 © 2015 Federation of European Biochemical Societies ;Copyright © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. ;ISSN: 0014-5793 ;EISSN: 1873-3468 ;DOI: 10.1016/j.febslet.2012.02.045 ;PMID: 22569257

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Title:
TLR-induced NF-κB activation regulates NLRP3 expression in murine macrophages
Author: Qiao, Yu ; Wang, Peng ; Qi, Jianni ; Zhang, Lei ; Gao, Chengjiang
Subjects: Animals ; Carrier Proteins - genetics ; Carrier Proteins - metabolism ; Cell Line, Transformed ; Cells, Cultured ; CHIP ; chromatin immunoprecipitation ; DNA-Binding Proteins - metabolism ; electrophoretic mobility shift assay ; EMSA ; Gene Expression Regulation - drug effects ; Lipopolysaccharides ; Macrophage ; Macrophage Activation - drug effects ; Macrophages - cytology ; Macrophages - drug effects ; Macrophages - immunology ; Macrophages - metabolism ; Macrophages, Peritoneal - cytology ; Macrophages, Peritoneal - drug effects ; Macrophages, Peritoneal - immunology ; Macrophages, Peritoneal - metabolism ; Mice ; Mice, Inbred C57BL ; Mutant Proteins - antagonists & inhibitors ; Mutant Proteins - metabolism ; NF-kappa B - antagonists & inhibitors ; NF-kappa B - genetics ; NF-kappa B - metabolism ; NF-κB ; NLR Family, Pyrin Domain-Containing 3 Protein ; NLRP3 ; NOD-LRRs containing pyrin domain 3 ; nuclear factor kappa B ; nucleotide ; Promoter Regions, Genetic - drug effects ; Recombinant Proteins - antagonists & inhibitors ; Recombinant Proteins - metabolism ; Response Elements - drug effects ; RNA, Messenger - metabolism ; TLR ; Toll-like receptor ; Toll-Like Receptors - agonists ; Toll-Like Receptors - metabolism ; Transcription Factor RelA - antagonists & inhibitors ; Transcription Factor RelA - genetics ; Transcription Factor RelA - metabolism
Is Part Of: FEBS letters, 2012-04, Vol.586 (7), p.1022-1026
Description: ► NLRP3 expression was induced by TLR stimulation in a NF-κB-dependent manner in murine macrophages. ► The NF-κB binding elements in NLRP3 promoter were identified. ► NF-κB binding to NLRP3 promoter was confirmed with EMSA and ChIP assays in LPS-stimulated macrophages. NLRP3 inflammasome plays a critical role in the activation of caspase-1 and maturation of IL-1β. However, the specific cis- and trans-regulatory elements that determine the extent of NLRP3 expression are not well defined. In this study, we found NLRP3 expression was induced by TLR agonists in murine macrophages in a NF-κB dependent manner. Furthermore, the corresponding NF-κB binding sites (nt −1303 to −1292 and −1238 to −1228) were identified in the NLRP3 promoter. Finally, EMSA and ChIP assays demonstrated LPS-induced NF-κB binding to the NLRP3 promoter. Therefore, out results delineated the molecular mechanisms involved in TLR-induced transcriptional regulation of NLRP3.
Publisher: England: Elsevier B.V
Language: English
Identifier: ISSN: 0014-5793
EISSN: 1873-3468
DOI: 10.1016/j.febslet.2012.02.045
PMID: 22569257
Source: Geneva Foundation Free Medical Journals at publisher websites
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TLR-induced NF-κB activation regulates NLRP3 expression in murine macrophages

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