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Differences in the endocannabinoid system of sperm from fertile and infertile men

PloS one, 2012-10, Vol.7 (10), p.e47704-e47704 [Peer Reviewed Journal]

COPYRIGHT 2012 Public Library of Science ;COPYRIGHT 2012 Public Library of Science ;Lewis et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. ;2012 Lewis et al 2012 Lewis et al ;ISSN: 1932-6203 ;EISSN: 1932-6203 ;DOI: 10.1371/journal.pone.0047704 ;PMID: 23082196

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Title:
Differences in the endocannabinoid system of sperm from fertile and infertile men
Author: Lewis, Sheena E M ; Rapino, Cinzia ; Di Tommaso, Monia ; Pucci, Mariangela ; Battista, Natalia ; Paro, Rita ; Simon, Luke ; Lutton, Deborah ; Maccarrone, Mauro
Vaudry, Hubert
Subjects: 2-Arachidonoylglycerol ; Acrosome reaction ; Adult ; Alcohols ; Anandamide ; Binding ; Biology ; Biomarkers ; Biosynthesis ; Cannabinoid CB2 receptors ; Cannabis ; Capacitation ; Capsaicin receptors ; Care and treatment ; Demography ; Drugs ; Endocannabinoid system ; Endocannabinoids ; Endocannabinoids - genetics ; Endocannabinoids - metabolism ; Enzyme-linked immunosorbent assay ; Exploitation ; Fatty acids ; Fatty-acid amide hydrolase ; Fertility ; Fertility - genetics ; Fertilization ; Gene expression ; Gene Expression Regulation ; Health aspects ; Humans ; Infertility ; Infertility, Male ; Infertility, Male - genetics ; Infertility, Male - metabolism ; Male ; Male infertility ; Marijuana ; Medicine ; Men ; Mens health ; Metabolism ; Motility ; mRNA ; Nervous system ; Phospholipase D ; Physiological aspects ; Physiology ; Polymerase chain reaction ; Public health ; Receptors ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Rodents ; Science ; Semen ; Semen - metabolism ; Sperm ; Spermatogenesis ; Spermatozoa ; Spermatozoa - metabolism ; Spermatozoa - pathology ; Tobacco ; Western blotting
Is Part Of: PloS one, 2012-10, Vol.7 (10), p.e47704-e47704
Description: Male infertility is a major cause of problems for many couples in conceiving a child. Recently, lifestyle pastimes such as alcohol, tobacco and marijuana have been shown to have further negative effects on male reproduction. The endocannabinoid system (ECS), mainly through the action of anandamide (AEA) and 2-arachidonoylglycerol (2-AG) at cannabinoid (CB(1), CB(2)) and vanilloid (TRPV1) receptors, plays a crucial role in controlling functionality of sperm, with a clear impact on male reproductive potential. Here, sperm from fertile and infertile men were used to investigate content (through LC-ESI-MS), mRNA (through quantitative RT-PCR), protein (through Western Blotting and ELISA) expression, and functionality (through activity and binding assays) of the main metabolic enzymes of AEA and 2-AG (NAPE-PLD and FAAH, for AEA; DAGL and MAGL for 2-AG), as well as of their binding receptors CB(1), CB(2) and TRPV1. Our findings show a marked reduction of AEA and 2-AG content in infertile seminal plasma, paralleled by increased degradation: biosynthesis ratios of both substances in sperm from infertile versus fertile men. In addition, TRPV1 binding was detected in fertile sperm but was undetectable in infertile sperm, whereas that of CB(1) and CB(2) receptors was not statistically different in the two groups. In conclusion, this study identified unprecedented alterations of the ECS in infertile sperm, that might impact on capacitation and acrosome reaction, and hence fertilization outcomes. These alterations might also point to new biomarkers to determine male reproductive defects, and identify distinct ECS elements as novel targets for therapeutic exploitation of ECS-oriented drugs to treat male fertility problems.
Publisher: United States: Public Library of Science
Language: English
Identifier: ISSN: 1932-6203
EISSN: 1932-6203
DOI: 10.1371/journal.pone.0047704
PMID: 23082196
Source: GFMER Free Medical Journals
MEDLINE
PubMed Central
Public Library of Science (PLoS)
ProQuest Central
DOAJ Directory of Open Access Journals

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Differences in the endocannabinoid system of sperm from fertile and infertile men

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