Language:

Prostate extracellular vesicles in patient plasma as a liquid biopsy platform for prostate cancer using nanoscale flow cytometry

Oncotarget, 2016-02, Vol.7 (8), p.8839-8849

ISSN: 1949-2553 ;EISSN: 1949-2553 ;DOI: 10.18632/oncotarget.6983 ;PMID: 26814433

Full text available

Citations Cited by

Actions
1. Add to My Research
2. Remove from My Research
3. E-mail
4. Print
5. Permalink
6. Citation
7. EasyBib
8. EndNote
9. RefWorks
10. Delicious
11. Export RIS
12. Export BibTeX

Title:
Prostate extracellular vesicles in patient plasma as a liquid biopsy platform for prostate cancer using nanoscale flow cytometry
Author: Biggs, Colleen N ; Siddiqui, Khurram M ; Al-Zahrani, Ali A ; Pardhan, Siddika ; Brett, Sabine I ; Guo, Qiu Q ; Yang, Jun ; Wolf, Philipp ; Power, Nicholas E ; Durfee, Paul N ; MacMillan, Connor D ; Townson, Jason L ; Brinker, Jeffrey C ; Fleshner, Neil E ; Izawa, Jonathan I ; Chambers, Ann F ; Chin, Joseph L ; Leong, Hon S
Subjects: 60 APPLIED LIFE SCIENCES ; Adult ; Antibodies, Monoclonal - immunology ; Biopsy ; Case-Control Studies ; Cell Biology ; Cell-Derived Microparticles - metabolism ; Cell-Derived Microparticles - pathology ; Extracellular Vesicles - metabolism ; Extracellular Vesicles - pathology ; Flow Cytometry - methods ; Follow-Up Studies ; Humans ; Male ; Microscopy, Atomic Force ; Nanotechnology ; Neoplasm Grading ; Neoplasm Staging ; Oncology ; Prognosis ; Prostate - metabolism ; Prostate - pathology ; Prostate - surgery ; Prostate-Specific Antigen - blood ; Prostatectomy ; Prostatic Hyperplasia - blood ; Prostatic Hyperplasia - pathology ; Prostatic Hyperplasia - surgery ; Prostatic Neoplasms - blood ; Prostatic Neoplasms - pathology ; Prostatic Neoplasms - surgery ; Prostatic Neoplasms, Castration-Resistant - blood ; Prostatic Neoplasms, Castration-Resistant - pathology ; Prostatic Neoplasms, Castration-Resistant - surgery ; Proteasome Endopeptidase Complex - immunology ; Retrospective Studies ; Tumor Cells, Cultured ; Young Adult
Is Part Of: Oncotarget, 2016-02, Vol.7 (8), p.8839-8849
Description: Extracellular vesicles released by prostate cancer present in seminal fluid, urine, and blood may represent a non-invasive means to identify and prioritize patients with intermediate risk and high risk of prostate cancer. We hypothesize that enumeration of circulating prostate microparticles (PMPs), a type of extracellular vesicle (EV), can identify patients with Gleason Score≥4+4 prostate cancer (PCa) in a manner independent of PSA. Plasmas from healthy volunteers, benign prostatic hyperplasia patients, and PCa patients with various Gleason score patterns were analyzed for PMPs. We used nanoscale flow cytometry to enumerate PMPs which were defined as submicron events (100-1000nm) immunoreactive to anti-PSMA mAb when compared to isotype control labeled samples. Levels of PMPs (counts/µL of plasma) were also compared to CellSearch CTC Subclasses in various PCa metastatic disease subtypes (treatment naïve, castration resistant prostate cancer) and in serially collected plasma sets from patients undergoing radical prostatectomy. PMP levels in plasma as enumerated by nanoscale flow cytometry are effective in distinguishing PCa patients with Gleason Score≥8 disease, a high-risk prognostic factor, from patients with Gleason Score≤7 PCa, which carries an intermediate risk of PCa recurrence. PMP levels were independent of PSA and significantly decreased after surgical resection of the prostate, demonstrating its prognostic potential for clinical follow-up. CTC subclasses did not decrease after prostatectomy and were not effective in distinguishing localized PCa patients from metastatic PCa patients. PMP enumeration was able to identify patients with Gleason Score ≥8 PCa but not patients with Gleason Score 4+3 PCa, but offers greater confidence than CTC counts in identifying patients with metastatic prostate cancer. CTC Subclass analysis was also not effective for post-prostatectomy follow up and for distinguishing metastatic PCa and localized PCa patients. Nanoscale flow cytometry of PMPs presents an emerging biomarker platform for various stages of prostate cancer.
Publisher: United States: Impact Journals
Language: English
Identifier: ISSN: 1949-2553
EISSN: 1949-2553
DOI: 10.18632/oncotarget.6983
PMID: 26814433
Source: Geneva Foundation Free Medical Journals at publisher websites
MEDLINE
PubMed Central
Alma/SFX Local Collection

Back to results list


INSPIRE LIBRARY - TON DUC THANG UNIVERSITY	(84-028) 37 755 057	Feedback
19 Nguyen Huu Tho St. Dist.7, HCM	thuvien@tdtu.edu.vn	Feedback

Prostate extracellular vesicles in patient plasma as a liquid biopsy platform for prostate cancer using nanoscale flow cytometry

ISSN: 1949-2553 ;EISSN: 1949-2553 ;DOI: 10.18632/oncotarget.6983 ;PMID: 26814433

Searching Remote Databases, Please Wait