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Development and application of glyco-bioinformatics packages for targeted quantitation

open access ;ISBN: 9789462957237 ;ISBN: 9462957231

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  • Title:
    Development and application of glyco-bioinformatics packages for targeted quantitation
  • Author: Jansen, B.C.
  • Subjects: Bioinformatics ; Chromatography ; Glycomics ; Glycoproteomics ; Mass spectrometry ; Pregnancy ; Rheumatoid arthritis
  • Description: This dissertation describes the development of glyco-bioinformatics tools that facilitate the high-throughput data processing of glycomics and glycoproteomics experiments, specifically for both MALDI-TOF-MS (Chapter 2) and LC-ESI-MS (Chapter 3). The developed methods also provide various quality control parameters that assist the researcher in curating both the measured spectra and quantified analytes, thereby providing high-quality data in a high-throughput manner.The tools that were developed within this thesis have been used to identify the influence of glycosylation on trypsin efficacy of Immunoglobulin G (Chapter 3) and two biological cohorts. Specifically, to investigate the serum N-glycosylation during and after pregnancy (Chapter 5) and to identify the differences in the N-glycosylation between maternal and fetal serum and IgG (Chapter 6).
  • Creation Date: 2017
  • Language: English
  • Identifier: ISBN: 9789462957237
    ISBN: 9462957231
  • Source: Leiden University Repository
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