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Mutagenicity and DNA Damage of Bisphenol a and its Structural Analogues in Hepg2 Cells

Arhiv za higijenu rada i toksikologiju, 2013-06, Vol.64 (2), p.3-14 [Peer Reviewed Journal]

ISSN: 0004-1254 ;EISSN: 1848-6312 ;DOI: 10.2478/10004-1254-64-2013-2319 ;PMID: 23819927 ;CODEN: AHRTAN

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  • Title:
    Mutagenicity and DNA Damage of Bisphenol a and its Structural Analogues in Hepg2 Cells
  • Author: Fic, Anja ; Žegura, Bojana ; Sollner Dolenc, Marija ; Filipič, Metka ; Peterlin Mašič, Lucija
  • Subjects: Ames test ; Amesov test ; Animals ; Benzhydryl Compounds - chemistry ; Benzhydryl Compounds - classification ; Benzhydryl Compounds - toxicity ; bisfenoli ; bisphenols ; Cell Line ; comet assay ; Cytotoxins - chemistry ; Cytotoxins - classification ; Cytotoxins - toxicity ; DNA Damage ; genotoxicity ; Hep G2 Cells ; Humans ; kometni test ; Male ; Molecular Structure ; Mutagenicity Tests ; Mutagens - chemistry ; Mutagens - toxicity ; Phenols - chemistry ; Phenols - classification ; Phenols - toxicity ; Rats ; Salmonella typhimurium ; Structure-Activity Relationship
  • Is Part Of: Arhiv za higijenu rada i toksikologiju, 2013-06, Vol.64 (2), p.3-14
  • Description: Environmental oestrogen bisphenol A (BPA) and its analogues are widespread in our living environment. Because their production and use are increasing, exposure of humans to bisphenols is becoming a significant issue. We evaluated the mutagenic and genotoxic potential of eight BPA structural analogues (BPF, BPAF, BPZ, BPS, DMBPA, DMBPS, BP-1, and BP-2) using the Ames and comet assay, respectively. None of the tested bisphenols showed a mutagenic effect in Salmonella typhimurium strains TA98 and TA100 in either the presence or absence of external S9-mediated metabolic activation (Aroclor 1254-induced male rat liver). Potential genotoxicity of bisphenols was determined in the human hepatoma cell line (HepG2) at non-cytotoxic concentrations (0.1 μmol L to 10 μmol L ) after 4-hour and 24-hour exposure. In the comet assay, BPA and its analogue BPS induced significant DNA damage only after the 24-hour exposure, while analogues DMBPS, BP-1, and BP-2 induced a transient increase in DNA strand breaks Okoljski estrogen, bisfenol A (BPA), in njegovi strukturni analogi so v veliki meri prisotni v našem okolju. Ker njihova proizvodnja in uporaba naraščata, je vse pomembneje ovrednotiti njihovo toksičnost zaradi izpostavljenosti ljudem. Z Amesovim in kometnim testom smo ovrednotili mutagenost in genotoksičnost osmih strukturnih analogov BPA (BPF, BPAF, BPZ, BPS, DMBPA, DMBPS, BP-1 in BP-2). Nobeden od testiranih bisfenolov ni izkazoval mutagenega delovanja na sevih TA98 in TA100 Salmonelle tryhimurium v prisotnosti in odsotnosti metabolne aktivacije (z Aroklorom 1254 inducirani encimi podganjih jeter). Potencialno genotoksičnost pa smo določali s kometnim testom na celični liniji humanega hepatoma (HepG2) pri necitotoksičnih koncentracijah (0.1 μmol L-1 do 10 μmol L-1) po 4-urni in 24-urni izpostavljenosti. BPA in njegov analog BPS sta pri kometnem testu povzročila poškodbe DNA samo po 24-urni izpostavljenosti, medtem ko so analogi DMBPS, BP-1 in BP-2 povzročili prehodne poškodbe DNA (samo po 4-urni izpostavljenosti). BPF, BPAF, BPZ in DMBPA niso povzročili poškodb DNA.
  • Publisher: Croatia: Versita
  • Language: English;Croatian;Slovenian
  • Identifier: ISSN: 0004-1254
    EISSN: 1848-6312
    DOI: 10.2478/10004-1254-64-2013-2319
    PMID: 23819927
    CODEN: AHRTAN
  • Source: DOAJ Directory of Open Access Journals
    AUTh Library subscriptions: ProQuest Central
    MEDLINE
    Walter De Gruyter: Open Access Journals
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