skip to main content
Language:
Search Limited to: Search Limited to: Resource type Show Results with: Show Results with: Search type Index

Adaptation of metal and antibiotic resistant traits in novel [beta]-Proteobacterium Achromobacter xylosoxidans BHW-15

PeerJ (San Francisco, CA), 2019-03, Vol.7, p.e6537 [Peer Reviewed Journal]

COPYRIGHT 2019 COPYRIGHT 2012-2015 PeerJ, Inc / Public user content licensed CC-BY 4.0 unless otherwise specified. Content is available for free at https://peerj.com ;COPYRIGHT 2019 PeerJ. Ltd. ;ISSN: 2167-8359 ;EISSN: 2167-8359 ;DOI: 10.7717/peerj.6537

Full text available

Citations Cited by
  • Title:
    Adaptation of metal and antibiotic resistant traits in novel [beta]-Proteobacterium Achromobacter xylosoxidans BHW-15
  • Author: Istiaq, Arif ; Shuvo, Md. Sadikur Rahman ; Rahman, Khondaker Md. Jaminur ; Siddique, Mohammad Anwar ; Hossain, M. Anwar ; Sultana, Munawar
  • Subjects: Aminoglycosides ; Analysis ; Aquatic ecosystems ; Arsenic ; Arsenic compounds ; Bacteria ; Bacterial genetics ; Cefepime ; Cephalosporins ; Disease susceptibility ; DNA sequencing ; Drug resistance ; Fluorides ; Genes ; Genomes ; Genomics ; Lactams ; Metals (Materials) ; Microbial drug resistance ; Novels ; Nucleotide sequencing ; Oxacillin ; Tetracycline ; Tetracyclines ; Transposons ; Water
  • Is Part Of: PeerJ (San Francisco, CA), 2019-03, Vol.7, p.e6537
  • Description: Chromosomal co-existence of metal and antibiotic resistance genes in bacteria offers a new perspective to the bacterial resistance proliferation in contaminated environment. In this study, an arsenotrophic bacterium Achromobacter xylosoxidans BHW-15, isolated from Arsenic (As) contaminated tubewell water in the Bogra district of Bangladesh, was analyzed using high throughput Ion Torrent Personal Genome Machine (PGM) complete genome sequencing scheme to reveal its adaptive potentiality. The assembled draft genome of A. xylosoxidans BHW-15 was 6.3 Mbp containing 5,782 functional genes, 1,845 pseudo genes, and three incomplete phage signature regions. Comparative genome study suggested the bacterium to be a novel strain of A. xylosoxidans showing significant dissimilarity with other relevant strains in metal resistance gene islands. A total of 35 metal resistance genes along with arsenite-oxidizing aioSXBA, arsenate reducing arsRCDAB, and mercury resistance merRTPADE operonic gene cluster and 20 broad range antibiotic resistance genes including [beta]-lactams, aminoglycosides, and multiple multidrug resistance (MDR) efflux gene complex with a tripartite system OM-IM-MFP were found co-existed within the genome. Genomic synteny analysis with reported arsenotrophic bacteria revealed the characteristic genetic organization of ars and mer operonic genes, rarely described in [beta]-Proteobacteria. A transposon Tn21 and mobile element protein genes were also detected to the end of mer (mercury) operonic genes, possibly a carrier for the gene transposition. In vitro antibiotic susceptibility assay showed a broad range of resistance against antibiotics belonging to [beta]-lactams, aminoglycosides, cephalosporins (1st, 2nd, and 3rd generations), monobactams and even macrolides, some of the resistome determinants were predicted during in silico analysis. KEGG functional orthology analysis revealed the potential of the bacterium to utilize multiple carbon sources including one carbon pool by folate, innate defense mechanism against multiple stress conditions, motility, a proper developed cell signaling and processing unit and secondary metabolism-combination of all exhibiting a robust feature of the cell in multiple stressed conditions. The complete genome of the strain BHW-15 stands as a genetic basis for the evolutionary adaptation of metal and the antibiotic coexistence phenomenon in an aquatic environment.
  • Publisher: PeerJ. Ltd
  • Language: English
  • Identifier: ISSN: 2167-8359
    EISSN: 2167-8359
    DOI: 10.7717/peerj.6537
  • Source: Geneva Foundation Free Medical Journals at publisher websites
    PubMed Central
    ROAD: Directory of Open Access Scholarly Resources
    ProQuest Central
    DOAJ Directory of Open Access Journals
Back to results list
INSPIRE LIBRARY - TON DUC THANG UNIVERSITY (84-028) 37 755 057 Feedback
19 Nguyen Huu Tho St. Dist.7, HCM thuvien@tdtu.edu.vn

Copyright © 2017 INSPIRE LIBRARY - TON DUC THANG UNIVERSITY

Searching Remote Databases, Please Wait