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Frameshift mutation in myostatin gene by zinc-finger nucleases results in a significant increase in muscle mass in Meishan sows

Czech Journal of Animal Science, 2020-01, Vol.65 (5), p.182-191 [Peer Reviewed Journal]

2020. This work is published under https://www.agriculturejournals.cz/web/about/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. ;ISSN: 1212-1819 ;EISSN: 1805-9309 ;DOI: 10.17221/265/2019-CJAS

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  • Title:
    Frameshift mutation in myostatin gene by zinc-finger nucleases results in a significant increase in muscle mass in Meishan sows
  • Author: Bi, Hanfang ; Xie, Shanshan ; Cai, Chunbo ; Qian, Lili ; Jiang, Shengwang ; Xiao, Gaojun ; Li, Biao ; Li, Xiang ; Cui, Wentao
  • Subjects: Cloning ; Frameshift mutation ; Hypertrophy ; lean meat yield ; Meat ; mstn ; Muscles ; Musculoskeletal system ; Mutation ; myofibres ; Myostatin ; Nuclease ; Nucleotides ; Skeletal muscle ; Swine ; Technology ; zfn-edited ; Zinc ; Zinc finger proteins
  • Is Part Of: Czech Journal of Animal Science, 2020-01, Vol.65 (5), p.182-191
  • Description: Myostatin (MSTN) is a negative regulator of skeletal muscle growth and development. A significant increase in skeletal muscle was observed in Mstn−/− mice compared with wild-type mice. So far, there has been no report on porcine MSTN mutations leading to skeletal muscle hypertrophy. In this report a MSTN frameshift mutation missing 11 nucleotides in exon 2 was introduced into Meishan pigs by zinc finger nuclease (ZFN) technology. ZFN-edited MSTN−/− Meishan pigs were successfully produced by a cloning method of somatic cell nucleus transfer. Results from slaughter experiments indicated that lean meat yield increased 16.53% in about 80 kg (10-months-old) MSTN−/− Meishan sows compared with their corresponding wild-type counterparts. The lean percentage of carcass from MSTN−/− sows was 61.20% vs 48.25% for MSTN+/− sows and 44.67% for wild-type sows. The fat of MSTN−/− sows was significantly lower than that of MSTN+/− and wild-type sows. The loin eye area of MSTN−/− Meishan sows (56.42 cm2) was greater than that of MSTN+/− (37.39 cm2) and wild-type (26.26 cm2) sows. The muscle fibre area of longissimus muscle in wild-type Meishan sows (1 946 μm2) was significantly greater than that of MSTN+/− (1 324 μm2) and MSTN−/− (1 419 μm2) sows. Moreover the significantly increased skeletal muscle in these MSTN−/− Meishan sows was mainly due to the increase in the number of myofibres rather than to hypertrophy. Compared with wild-type Meishan sows, it was noted that myofibres had transformed from type I to IIB in MSTN−/− Meishan sows. Our present study demonstrated that frameshift mutation in MSTN by ZFN technology led to a significant increase in muscle mass and a significant decrease in fat content in Meishan sows.
  • Publisher: Prague: Czech Academy of Agricultural Sciences (CAAS)
  • Language: English;Czech;Slovak
  • Identifier: ISSN: 1212-1819
    EISSN: 1805-9309
    DOI: 10.17221/265/2019-CJAS
  • Source: DOAJ : Directory of Open Access Journals
    AUTh Library subscriptions: ProQuest Central
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