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Ropivacaine promotes apoptosis of hepatocellular carcinoma cells through damaging mitochondria and activating caspase-3 activity

Biological research, 2019-07, Vol.52 (1), p.36-10, Article 36 [Peer Reviewed Journal]

COPYRIGHT 2019 BioMed Central Ltd. ;The Author(s) 2019 ;This work is licensed under a Creative Commons Attribution 4.0 International License. ;ISSN: 0717-6287 ;ISSN: 0716-9760 ;EISSN: 0717-6287 ;DOI: 10.1186/s40659-019-0242-7 ;PMID: 31300048

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  • Title:
    Ropivacaine promotes apoptosis of hepatocellular carcinoma cells through damaging mitochondria and activating caspase-3 activity
  • Author: Wang, Wenting ; Zhu, Mingyue ; Xu, Zhixin ; Li, Wei ; Dong, Xu ; Chen, Yi ; Lin, Bo ; Li, Mengsen
  • Subjects: Apoptosis ; BIOLOGY ; Cancer ; Cancer cells ; Cancer research ; Carcinoma ; Caspase-3 activity ; Caspases ; Cellular signal transduction ; Computer simulation ; Cytochrome c ; Drug therapy ; Electron microscopy ; Enzymes ; Health aspects ; Hepatocellular carcinoma ; Hepatocellular carcinoma cells ; Local anaesthetic agent ; Local anesthetics ; Microscopy ; Mitochondria ; Novels ; Proteins ; Signal transduction
  • Is Part Of: Biological research, 2019-07, Vol.52 (1), p.36-10, Article 36
  • Description: Recent evidences indicated that some local anaesthetic agents played a role in inhibiting the proliferation of cancer cells; Whether ropivacaine is able to promote apoptosis of hepatocellular carcinoma (HCC) cells is still unclear. The aim of this study was to investigate the effect of ropivacaine on the apoptosis of HCC cells. In the present study, we treated the HCC cell lines, Bel7402 and HLE with ropivacaine. MTT, DAPI stain, trypan blue exclusion dye assay, flow cytometry, electron microscopy, computational simulation, laser confocal microscope, Western blotting, and enzyme activity analysis of caspase-3 were applied to detect the growth and apoptosis of HCC cells and to explore the role mechanism of ropivacaine. Ropivacaine was able to inhibit proliferation and promote apoptosis of HCC cells in a dose- and time-dependent manner. Ropivacaine also has a trait to inhibit the migration of HCC cells; ropivacaine damaged the mitochondria of HCC cells. The results also indicated that ropivacaine was able to interact with caspase-3, promote cytoplasmic caspase-3 migration into the nucleus, stimulate cleavage of caspase-3 and PARP-1, caspase-9 proteins, inhibit the expression of Bcl-2, promote expression of Apaf-1 and mitochondria release cytochrome C, and activate the activity of caspase-3. Ropivacaine has a novel role in promoting apoptosis of HCC cells; The role mechanism of ropivacaine maybe involve in damaging the function of mitochondria and activating the caspase-3 signalling pathway in HCC cells. Our findings provide novel insights into the local anaesthetic agents in the therapy of HCC patients.
  • Publisher: England: BioMed Central Ltd
  • Language: English;Portuguese
  • Identifier: ISSN: 0717-6287
    ISSN: 0716-9760
    EISSN: 0717-6287
    DOI: 10.1186/s40659-019-0242-7
    PMID: 31300048
  • Source: SciELO
    Geneva Foundation Free Medical Journals at publisher websites
    PubMed Central
    Springer OA刊
    ProQuest Central
    DOAJ Directory of Open Access Journals
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