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In Vitro Propagation of the Dendrobium anosmum Lindl. Collected in Vietnam

Agronomy (Basel), 2022-02, Vol.12 (2), p.324 [Peer Reviewed Journal]

2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. ;ISSN: 2073-4395 ;EISSN: 2073-4395 ;DOI: 10.3390/agronomy12020324

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  • Title:
    In Vitro Propagation of the Dendrobium anosmum Lindl. Collected in Vietnam
  • Author: Nguyen, Hai T. ; Dinh, Son T. ; Ninh, Thao T. ; Nong, Hue T. ; Dang, Tam T. T. ; Khuat, Quyet V. ; Dang, Anh T. P. ; Ly, My T. ; Kirakosyan, Rima N. ; Kalashnikova, Elena A.
  • Subjects: Activated carbon ; Activated charcoal ; Cell division ; Charcoal ; Culture ; Dendrobium ; Dendrobium anosmum ; Efficiency ; Experiments ; Genetic resources ; Growth regulators ; in vitro ; Kinetin ; micropropagation ; pH effects ; Plant tissues ; Propagation ; protocorm ; Regeneration ; Seeds ; Sucrose ; Tissue culture ; Vietnam
  • Is Part Of: Agronomy (Basel), 2022-02, Vol.12 (2), p.324
  • Description: Hoa Binh province is one of the best places for orchids in Vietnam. The climate and environment of Hoa Binh province are favorable for the development of orchids, especially rare indigenous ones. Dendrobium anosmum Lindl., which stands out because of the unique fragrance and colors, is one of the most popular varieties in Hoa Binh province. To meet the increasing demands of the industrial market as well as to contribute to the preservation and development of genetic resources of Dendrobium sp. in Hoa Binh province, propagating D. anosmum Lindl. is a crucial step. Plant tissue culture, which has been applied to improve reproducibility of orchids for many years, is still an effective method, especially for large-scale propagation. Studies on in vitro propagation of D. anosmum Lindl. from Hoa Binh province showed that growth regulators (BA, kinetin, α-NAA) did not have a significant effect on protocorm initiation because D. anosmum Lind. from Hoa Binh province already has a high rate of regeneration. However, MS medium + 1.0 mg/L kinetin + 0.5 mg/L α-NAA + 30 g sucrose + 8.0 g agar per liter, pH 5.7–5.8 was the optimal medium to increase shoot length. The MS medium + 1.0 g activated charcoal + 30 g sucrose + 8.0 g agar per liter, pH 5.7–5.8 was the most suitable medium for shoot growth—after 6 weeks of culture, the average shoot length was 1.09 cm, the average number of leaves was 6.13, the average number of roots was 3.17, and the average root length was 1.11 cm—about 3.3, 4.17, 3.41, and 1.67 times higher, respectively, than in the control (without activated charcoal).
  • Publisher: Basel: MDPI AG
  • Language: English
  • Identifier: ISSN: 2073-4395
    EISSN: 2073-4395
    DOI: 10.3390/agronomy12020324
  • Source: ROAD: Directory of Open Access Scholarly Resources
    ProQuest Central
    DOAJ Directory of Open Access Journals

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